Effect Of Quercetagetin On Growth Performance,Immune And Antioxidant Related Indexes In Broilers

The purpose of the study was to evaluate the effects of quercetagetin on broiler growth performance,immunity and antioxidation.A total of 400 1-day-old Ross 308 broilers were selected and randomly divided into 4 groups,with 10 replicates in each group,and 10 broilers in each replicate.Broilers in control group were fed a basal diet,and the others in experimental groups were fed the basal diets supplemented with 3.2 mg/kg,4.8 mg/kg and 6.4 mg/kg quercetagetin(3.2 mg/kg,4.8 mg/kg and 6.4 mg/kg quercetagetin group).The trial period was 42 days,with 1 to 21 days in the early period and 22 to 42 days in the later period.The results showed that:1)The effects of quercetagetin on the growth performance of broilers are as follows.Compared with the control group,adding 3.2 mg/kg quercetagetin to the diet can significantly increase the 21-day-old body weight and the average daily gain of broilers(P<0.05).Adding 3.2 mg/kg and 4.8 mg/kg quercetagetin to the diet can significantly reduce the broiler feed-to-weight ratio of 1 to 21 days(P<0.05).Adding 4.8 mg/kg quercetagetin to the diet can significantly increase the average daily gain of 22 to 42 days of broilers(P<0.05),and significantly reduce the feed to gain ratio of broilers(P<0.05);Adding 6.4 mg/kg quercetagetin to the diet can significantly increase the apparent metabolic rate of 21-day crude fat(EE)and 42-day crude protein(CP)of broilers(P<0.05).Adding 4.8 mg/kg and 6.4 mg/kg quercetagetin to the diet can significantly increase the apparent metabolic rate of 42-day crude phosphorus(P)of broilers(P<0.05);Adding 4.8 mg/kg quercetagetin to the diet can significantly increase the semi-eviscerated rate of broilers(P<0.05);Adding 6.4 mg/kg quercetagetin to the diet can significantly increase the total evisceration rate of broilers(P<0.05);Adding 4.8 mg/kg and 6.4 mg/kg quercetagetin to the diet can significantly increase liver index of broilers(P<0.05);Adding 4.8 mg/kg quercetagetin to the diet can significantly increase the height of villi of jejunum and ileum of broilers(P<0.05).Adding quercetagetin to the diet can significantly reduce the crypt depth of jejunum and ileum of broilers(P<0.05),and significantly increase the villus height/crypt depth of jejunum and ileum of broilers(P<0.05);Adding quercetagetin to the diet can significantly increase the serum total protein content of broilers(P<0.05).2)The effects of quercetagetin on the immune indices of broilers are as follows.Compared with the control group,on d 21,adding quercetagetin to the diet can significantly increase the content of white blood cells(WBC),red blood cells(RBC)and hemoglobin(HGB)of broilers(P<0.05);Adding 3.2 mg/kg and 4.8 mg/kg quercetagetin to the diet can significantly increase the serum complement 4(C4)content(P<0.05)of broilers.On d 21,adding 4.8 mg/kg and 6.4 mg/kg quercetagetin to the diet can significantly increase the serum immunoglobulin M(IgM)content of broilers(P<0.05).On d 42,adding quercetagetin to the diet can significantly increase the serum immunoglobulin G(IgG)content of broilers(P<0.05).3)The effects of quercetagetin on the antioxidant indices of broilers are as follows.Compared with the control group,adding 3.2 mg/kg quercetagetin to the diet can significantly increase the total antioxidant capacity(T-AOC)of serum of broilers(P<0.05).On d 21,adding 6.4 mg/kg quercetagetin to the diet can significantly increase the activity of serum superoxide dismutase(SOD)of broilers(P<0.05).On d 42,adding quercetagetin to the diet can significantly increase the activity of serum SOD of broilers(P<0.05);Adding 4.8 mg/kg quercetagetin to the diet can significantly increase the T-AOC of the jejunal mucosa of broilers(P<0.05).Adding 4.8 mg/kg and 6.4 mg/kg quercetagetin to the diet can significantly increase the T-AOC of the ileal mucosa of broilers(P<0.05);Adding 3.2 mg/kg and 6.4 mg/kg quercetagetin to the diet can significantly reduce the malondialdehyde(MDA)content in the liver of broilers(P<0.05).Adding 6.4 mg/kg quercetin marigold can significantly increase the activity of SOD in the liver of broilers(P<0.05);Adding quercetagetin to the diet can significantly increase the expression of nuclear factor E2 related factor 2(Nrf2)gene mRNA in the liver of broilers(P<0.05).Adding 4.8 mg/kg and 6.4 mg/kg quercetagetin to the diet can significantly increase the mRNA expression level of the liver copper-zinc superoxide dismutase(SOD1)gene of broilers(P<0.05).In summary:the addition of quercetagetin in the feed can effectively improve the antioxidant performance of broilers by regulating the Keap1-Nrf2-ARE signaling pathway,and improve the immunity of broilers,and improve the intestinal tissue structure of broilers,and promote the digestion and absorption of nutrients in the intestine of broilers,thereby improving broilers growth performance.So quercetagetin can be better used in broiler production,and the recommended dosage is 3.2 to 4.8 mg/kg.