Asexual Propagation Technology Of Himalayan Cherry

Cerasus.cerasoides D.Don belongs to the Rosaceae Cerasus Mill.It is a tall deciduous tree with luxurious and elegant flower type.It blooms around October in Nepal,the country of origin.It has high ornamental value,strong tillering ability,disease and insect resistance and other excellent characteristics.This paper studied the asexual propagation technology of Himalayan cherry such as cutting and tissue culture.The cutting propagation experiment with the method of soft cutting,we were studied the effects of substrates and different parts of one stem on rooting rate,survival rate and growth of young sprouts.A comparative study on the differences of rooting and survival of Himalayan cherry with other seven cherry blossom varieties was studied,and the effects of applying fertilizer on the young shoot elongationand and the chlorophyll content of leaves for cutting seedlings of Himalayan cherry were testde.The buds of spring germination of cerasus himalaya were used as explants.Orthogonal experiment design was used to screen the medium components of adventitious bud proliferation and rooting of cerasus Himalayan.The effects of different plant growth regulators on callus induction were studied using the leaves of sterile seedlings as explants.The test results are as follows:(1)The results show that cultivation substrates significantly affect rooting,survival and growth of young sprouts.Cutting rooting rate reaches up to 92.0% in the fine river sand /perlite(1:1),and survival rate is 85.3% in peat soil/perlite(2:1),the average length of new sprouts can reach up to 11.5cm in peat soil/perlite(3:1)substrate.The effect of different parts of cuttings on rooting rate was significantly different,the results showed that the average rooting rate and survival rate of middle cuttings were the highest,reaching 97.3% and 92.7% respectively.The rooting rate of cuttings treated with germinal powder was as high as 99.3%,while that of cuttings not treated with germinal powder was 60.7%.(2)Results showed that there were significant differences among different cherry blossom varieties in rooting rate and survival rate,and Himalayan cherry cuttings occupied the highest rooting rate and survival rate,with 97.3% and 92.7%respectively,and had faster rooting process.Meanwhile,C.pseudocrasus ’Introrsa’had the lowest rooting rate of 46.0%,and C.campanulata ’Youkou’ had the survival rate of 0.From the results of fertilizing experiments on cutting seedlings of Himalayan cherry,it was found that application of nitrogen fertilizer can promote young shoot elongation and improve the chlorophyll content of leaves.However,the different fertilization concentrations affected significantly the length of young shoots and chlorophyll content of leaves of cutting seedlings,and the 1000 time liquid of urea showed the best effects on the young shoots elongation and leaf chlorophyll content for cutting seedlings.(3)The medium group suitable for the proliferation of adventitious buds of Himalaya cherry was divided into WPM + 6-BA 0.5mg /L,and the proliferation rate reached about 5.5.The optimal medium group for inducing adventitious bud rooting was divided into 1/2 MS + IBA 1.0mg /L + NAA 1.0mg /L.The transplanting and suitable for callus induction was 1/2 MS + 2,4-D 2.0 mg/L + 6-BA 0.5mg/L.This study established the techniques of cutting and tissue culture for rapid propagation of Himalaya cherry,which laid the foundation for large-scale asexual propagation of Himalaya cherry.