Design Of Miniaturized And Multimodal Microscopic Imaging System For Real-Time Detectiond

At present,the distribution of medical resources in China is uneven.In order to promote the development of primary medical institutions,it is of great significance to promote the development of point-of-care testing technology.As an intuitive detection tool,microscope often plays an important role in field detection.In existing applications,the microscope often has a single mode,and due to the limitation of biological conditions,the cell samples may not be stained.For the phase samples,the traditional bright field imaging often can’t obtain the ideal microscopic image.The application of dark field imaging or phase contrast imaging can enhance the contrast,and the observation effect is better than that of bright field.However,this kind of multi-mode microscope is often expensive and complex to operate,which can’t be borne by primary medical institutions.Therefore,designing a miniaturized multi-mode microscopic imaging system for on-site detection can improve the conditions of primary medical institutions and expand the application scope of on-site detection technology.In view of the above background,this paper carries out a series of work around multi-mode micro imaging technology and miniaturized instrument design.By analyzing the bright field imaging principle,dark field imaging principle,differential phase contrast imaging principle and phase imaging principle based on the equation of intensity,an optical path design scheme suitable for miniaturized multi-mode microscopic imaging system is obtained.According to the structure and core components of the commercial microscope,the device selection is carried out,and the mechanical structure that needs additional design is optimized,so that the miniaturized multi-mode microscopic imaging system can flexibly switch the imaging mode.The displacement mechanism in the system is adjusted by focusing ring,which is used to adjust the position of condenser lens,objective lens and camera respectively.The real-time acquisition of intensity image is realized by developing host computer software,and the computed imaging is realized by using the designed phase recovery algorithm.In order to expand the function of the system,the cell counting algorithm is added.The actual size of the final miniaturized multimode microscopic imaging system is about7.5×7.5×20cm,the overall weight is about 700 g.Based on the designed miniaturized multi-mode microscopic imaging system,the experimental test is carried out.Firstly,USAF1951 resolution plate is used to measure the resolution of the system under 10 times,20 times and 40 times of the objective lens.The resolution of the system under 10 x objective lens is 2.19 μm.the test results are better than the expected design objectives.Subsequently,based on a large number of biological samples,bright field imaging test,dark field imaging test,differential phase contrast imaging test and phase imaging test based on the equation of intensity were carried out respectively.The test results show that the miniaturized multi-mode micro imaging system designed in this paper can realize intensity imaging and phase imaging,and the time used for each mode test meets the field detection requirements.The device is low-cost and easy to operate,and provides an important real-time detection tool for primary medical institutions and field detection.