Research On Programmable Illumination Control And Multi-mode Light Field Imaging In Computational Imaging Microscope

The optical microscope is an indispensable observation instrument for life science research,and its technological innovation has profoundly affected the development of research fields such as biology,physics,chemistry,medicine,metallurgy,and brewing.Despite the tremendous advances in optical microscopy imaging,traditional imaging systems rely on fixed illumination and optical path detection architectures.Therefore,we still face the problem that information acquisition is limited by optical systems and detectors in terms of dimensions,functions,and performance,the problem that the mode of phase difference imaging is limited by physical hardware,the problem of difficulty to achieve "multi-mode" and fast mode switching for single device integration,and the problems that the field of view and the resolution cannot be effectively balanced.In view of the above problems,this paper focuses on the new theory and new methods of "computing microscopic imaging",and carries out research on programmable illumination control and multi-mode high-resolution optical field microscopy.We replaced the Kohler illumination source in a conventional microscope with a low-cost programmable LED array.The programmable LED array is manipulated by designing hardware driver circuits,PCB boards,software drivers,and optimizing lighting patterns to provide microscopic illumination with flexible angle and aperture.Thereby,the imaging modes such as bright field,dark field,differential phase contrast,Rheinberg,and rainbow dark field are realized in one system.This is based on the use of programmable LED arrays to provide street lighting at multiple angles.There is no need for any mechanical adjustment,just a set of images,we can digitally record a complete five-dimensional light field information.Using spatial domain projection integration and multi-mode imaging algorithms,brightfield,darkfield,differential phase contrast,and Rembrandt refocus microscopy can be achieved simultaneously,resulting in a high-resolution,fully-focused image.The proposed method shows the great potential in the development of future research fields such as biology,medicine,and life sciences.At the end of the paper,the above imaging modes were tested and evaluated using samples such as water cotton sample and butterfly mouth sample,which verified the reliability and accuracy of the research results.