Development Of A Multimodal Non-interferometric Quantitative Phase Microscope Based On Computational Optical Imaging

Optical microscopes have been widely used in medical and biological research,disease diagnosis,drug testing,and materials science since they were introduced more than 400 years ago.The optical microscope realizes the visualization of minute details,and can gain insight into the structure and function of the most diverse and smallest targets,leading human beings to make endless explorations in the endless micro-world.Especially in the fields of life sciences and biomedicine,optical microscopes have become indispensable tools for life science research,disease diagnosis and drug development.However,current optical microscopes are facing increasingly complex and expensive systems,and the observation of some biological samples can only be aided by staining or labeling.In view of the above problems,this thesis focuses on a new type of microscopic imaging method called "Computational Optical Imaging",and conducts research on multi-modal noninterference quantitative phase microscope.Use FPGA to drive programmable LED arrays to achieve high-speed refresh and flexible control of LED arrays;Differential phase contrast(DPC),a non-iterative phase recovery method in computational optical imaging,is used to achieve real-time quantitative phase imaging through GPU parallel computing acceleration.Use of background correction method based on inter-frame registration to correct non-uniform background in phase imaging;Based on the C ++ application framework Qt,the supporting microscope software is developed to perform automatic control of the microscope,calculation of phase imaging,and display and processing of images,and finally achieve the integration and one-click switching of multiple microscope imaging modes.In this thesis,the prototype development of the system is completed,and multi-modal microscopic imaging and quantitative phase imaging are realized.A set of computable imaging software has been developed,integrating functions of microscope control,image acquisition,phase calculation and result analysis.Finally,the performance of the system was verified by microscopic imaging of various biological samples and phase imaging of unstained He La cells and microlens arrays.