Study On The Performance Of Mesoporous Bioglass With Different Morphology And Constituent As Wound Dressing Material

Bioactive glass has good biocompatibility and can promote bone differentiation for its unique chemical components,so it has been used in hard tissue fields such as bone repair and tooth repair for a long time.In recent years,the application of bioactive glass in soft tissue repair has attracted more attentions.However,the amorphous structure and low specific surface area of bioactive glass,is not suitable to be used as a carrier of drugs.In this study,mesoporous bioactive glass(MBG)with spherical-like shape was prepared by organic template method,which increased the specific surface area of the material and makes it possible to become a drug carrier.On the basis of spherical-like mesoporous bioactive glass,the oval rod-like mesoporous bioactive glass was prepared by high temperature and high pressure,which can not only improve the specific surface area and pore volume,but also be more biocompatible.In addition,berberine hydrochloride was selected as the model drug in this study for its antibacterial,anti-inflammatory and hypoglycemic abilities.Comparison of biological properties of different mesoporous bioactive glasses is the main objective of this thesis.First,the spherical-like mesoporous bioglass(s MBG)was prepared by using cetyltrimethylammonium bromide as the organic template agent,ethyl orthosilicate(TEOS),triethyl phosphate(TEP)and calcium nitrate tetrahydrate(CN)as raw materials.Cu ions are added to the spherical-like mesoporous bioglass to prepare copper-carrying spherical-like bioglass(s MBG-Cu).Oval rod-like bioglass(o MBG)and copper-loaded oval rod-like bioglass(o MBG-Cu)were prepared on the basis of s MBG and s MBG-Cu by high-temperature and high-pressure treatment.Then the properties of the four mesoporous bioactive glasses prepared was characterized.The results showed that the addition of Cu ions not only promoted the migration of fibroblasts and the expression of vascularization factors of endothelial cells,but also Cu ions were beneficial to antibacterial properties of MBG.Under the condition of high temperature and high pressure,the spherical-like MBG morphology changed to oval rod-like MBG,which not only greatly increased the pore volume and specific surface area,but also promoted the migration of fibroblasts and the expression of endothelial cell vascularization related gene factors of VEGF,e NOS,and v WF.Secondly,the berberine hydrochloride drug was loaded on different mesoporous bioactive glasses in vitro,and it was determined that the berberine hydrochloride was loaded into the inner pores of the mesoporous bioactive glass.Because o MBG-Cu has a higher specific surface area and pore volume,it is better than the other three MBG materials in the drug encapsulation rate and loading rate,and the drug release in all MBGs can reach a longer period of more than 20 days.The addition of berberine hydrochloride is not only conducive to the expression of vascularization related genes,but also has inhibition of bacteria and antibacterial functions to a certain extent.The bacteriostatic effect of drug-loaded MBG is positively correlated with berberine hydrochloride content.Finally,the anti-inflammatory properties of the eight prepared mesoporous bioactive glasses were discussed and characterized by co-cultivation with macrophages RAW 264.7.The results show that the oval rod-like MBG has the higher degree function of inhibiting inflammation.With the addition of Cu ions of MBG,the secretion of IL-10 is reduced,indicating that Cu ions are not conducive to inhibiting inflammation.The secretion of IL-10 was increased greatly and the secretion of TNF-ɑ was reduced in those MBG groups which loaded berberine hydrochloride.Those results indicated that berberine hydrochloride is beneficial to anti-inflammatory.In general,after loaded berberine,the anti-inflammatory of mesoporous bioglass was improved.Therefore,this kind of berberine-loaded composite MBG material,which not only has antibacterial properties,promotes vascularization and good cytocompatibility,but also has anti-inflammatory properties,is expected to become a new wound dressing materials.