| Hyperthermophilic composting has the advantages of having a rapid temperature rise,a high maximum temperature,a short decomposition cycle and being complete harmlessness.These advantages can realize the efficient resource utilization of livestock and poultry manure.However,currently there are not many studies on Hyperthermophilic thermophilic bacteria for composting,even fewer reports on the preparation of Hyperthermophilic thermophilic bacterial products with good adaptability and functionality.In this study,strains that can efficiently degrade lignocellulose under Hyperthermophilic conditions were screened and identified from compost samples in the high-temperature period,and Hyperthermophilic thermophilic microbial agents were developed and inoculated into cow manure-straw Hyperthermophilic compost fermentation process to investigate their composting application effects.The main results obtained are as follows.1)Using 60℃ as the culture temperature,the hydrolysis circle test,filter paper strip disintegration,and straw degradation test were used for screening.Three Hyperthermophilic thermophilic strains with high cellulose degradation ability were selected from the high-temperature compost samples,and they were named as the straw degradation rates of strains L2,L8 and G1,L2,L8,and G1.After 15 days of cultivation,the degration rates were25.08%,23.16%,and 22.95%,respectively,which showed good straw degradation ability at high temperature.Molecular biology methods were used for identification:L2 strain was Ureibacillus terrenus,L8 strain was Geobacillus thermodenitrificans,and G1 strain was Thermoactinomyces Vulgaris.Studies on the growth characteristics of L2,L8,and G1strains were carried out to determine the growth characteristics of Hyperthermophilic thermophilic strains at different temperatures,p H,and salt concentrations.The optimum temperature for the growth of Hyperthermophilic thermophilic strains is 70℃,the optimum p H is 7.0,and the optimum salt concentration is 10.00 g·L-1～15.00 g·L-1.2)The best ratio of G1:L2:L8 was 1:4:3,and the cellulase activity of the mixture was42.73 U·m L-1.The GLL solid Hyperthermophilic thermophilic microbial agent was prepared by using grass carbon soil as the carrier.3)The application test results of GLL inoculant in cow manure+corn stalk.Hyperthermophilic composting showed that the high-temperature period （>50℃） of GLL treatment lasted for 18 days,and the Hyperthermophilic period （≥80℃） lasted for 5 days.On day 16,the GI value processed by GLL exceeds 80%.GLL treatment reduced the hemicellulose content in the compost by 81.60%,the cellulose content by 65.20%,and the lignin content by 53.70%.The microbial community structure analysis of samples at different composting periods confirmed that the GLL microbial agent had a promoting effect on the process of cow manure+corn stalk composting.The inspection of finished compost can basically meet the requirements in"Organic Fertilizer"（NY/T 525-2021）. |
