| In this study,the content of polyphenols,monophenols and evaluation of antioxidant capacity in vitro were determined from dry red and dry white wines of four producing areas of Xinjiang and Hebei were determined,and the correlation analysis was conducted.Meanwhile,Combined with animal experiments,the model SD health rats were gavaged for eight weeks,,physiological index,intestinal flora,liver cell autophagy related protein content of SD rats were determined,in order to explore the impact of wine nutrients to body health,providing theoretical basis for wine health industry development,the specific research results are as follows.1.The content of phenolic substances in wine were determined,the results were found that Cabernet Sauvignon dry red wine had more phenolic substances than Chardonnay dry white wine.The content of phenolic compounds increased gradually with the geographical location to the northwest.The content of phenols were positively correlated with antioxidant capacity in vitro.The polyphenols that contributed more to the antioxidant capacity were p-hydroxycinnamic acid,astragalus,p-hydroxybenzoic acid,flavanol-3-ol and flavonol in order.The monomer phenols that contributed more to oxidation resistance were erucic acid,salicylic acid,myricetin,catechin and epicatechin in order.2.The serum and liver biochemical indexes and the preparation of liver pathological sections of each group were determined,the results were found that long term moderate drinking wine can significantly improve the liver function,the blood lipid levels,and the antioxidant capacity of liver tissue.The blood nutrition index and renal function index were slightly improved.The beneficial substances of wine can reduce the adverse effects of alcohol on the body.Comparison of different experimental groups were found to reduce the body’s adverse reactions and polyphenols showed a positive correlation.3.There were no adverse effects on the growth and development of the rats in each group.the composition of intestinal flora was altered during long-term drinking of wine and increase the species and abundance of beneficial bacteria,such as Lactobacillus-Intestinalis,Odoribacter,Alistipes,etc.The correlation analysis between obesity index and different species was showed that body weight had a significant positive correlation with the Lactobacill-Intestinalis(P<0.05)and a significant negative correlation with the Lactobacilli(P<0.05).LDL-C was significantly positively correlated with Lachnospiraceae(P<0.05),and Glu was significantly positively correlated with Lactobacillus-Insomalis(P < 0.01).These beneficial bacteria genera are closely related to the body obesity index,wasfound that long-term wine consumption can increase the abundance of obesity-related intestinal microflora and thus improve the structure of intestinal microflora.4.The content of autophagy related protein was measured in liver of all samples.Compared with the control group,the protein expression of LC3 was significantly increased(P < 0.05),and the protein content of p62 was significantly decreased(P < 0.05)in wine group.Compared with the alcohol group,the contents of LC3 protein and p62 protein in the wine group were increased and decreased,respectively,and the two indexes in groups 1 and2 were significantly different from those in the alcohol group(P<0.05).Combined with liver indexes and liver sections,the liver function and histopathological damage were improved by comparing long-term drinking wine and drinking the same concentration of alcohol,suggesting that the beneficial substances of wine can protect the liver with a higher level than the control group.The beneficial components can reduce the liver injury of alcohol,which is consistent with literature reports,but the specific mechanism needs further research. |
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