Research On The Regulation Mechanism Of Engineered Microenvironment On Cell Growth

Cells in organisms are in a three-dimensional(3D)dynamic microenvironment.The migration,proliferation and differentiation of cells in the microenvironment are affected by many factors,such as the composition of extracellular matrix,cell-to-cell crosstalk,physical,chemical and biological signals generated by mechanical stimuli,etc.The 3D network structure formed by nano-sized collagen and elastin fibers in the extracellular matrix,and its interaction with the surrounding physical and chemical microenvironment all affect the function and behavior of cells.Therefore,the use of biomaterials with nanostructures to simulate the physical,chemical and biological signals in the extracellular microenvironment to regulate cell growth and function is a current research hotspot.This study intends to explore the impact of engineered microenvironment on cell behavior and function,and to analyze the regulatory mechanism of physical stimuli in affecting cell behavior.Specifically,the research was carried out from the following two aspects:1.In order to explore the role of physical stimulation in regulating cell biology,bionic 3D printing technology was used to prepare a biomimetic patch with high biocompatibility,which can provide a suitable microenvironment for cells.The combined effect of static magnetic field improves cell proliferation,adhesion and myogenic differentiation.Through in vitro and in vivo experiments,we have proved that the static magnetic field and regeneration patch can regulate myogenic differentiation by activating the p38? MAPK signaling pathway.Implanting the regeneration patch into the mouse muscle defect and exposing it to a suitable static magnetic field can enhance muscle regeneration.2.In order to simulate the growth microenvironment of natural pancreatic islets,we use porcine pancreas,liver and adipose tissue to make ECM hydrogel and construct 3D pancreatic islet tissue.The effect of hydrogels containing different ECMs on the remodeling of islet function was explored.Through drug testing,it is proved that the established 3D pancreatic islet tissue can be used for drug screening and blood sugar control,thereby contributing to disease research and treatment.This thesis aims to address the problems encountered in the process of tissue repair and modeling at this stage,using tissue engineering theory,combined with biomaterials with specific structures to simulate the extracellular microenvironment and using its physicochemical properties to control cell fate.Regeneration or modeling provides new ideas and theoretical foundations.