Effects Of Static Magnetic Fields On Islet Endothelial Cells And Angiogenesis

Angiogenesis is involved in multiple physiological and pathological processes including organ growth and repair,and regulated by a balance between pro-and anti-angiogenic signals,which plays a critical role in many diseases,such as cancer and diabetes.Angiogenesis has emerged as a novel target to prevent and treat these diseases,and many researchers focus on stimulation and inhibition of angiogenesis.Magnetic field is a common physical factor in our daily life.Experimental evidence suggests that magnetic fields with a certain parameter have biological effects on the organism and beneficial effects on the treatment of some diseases.Meanwhile,magnetobiology has attracted considerable amount of public concern,and the effect of magnetic fields on angiogenesis has also become one of the important study aspects.In this study,we took mouse islet microvascular endothelial cells(MS-1)as the research sample at the cellular level,and detected the changes in cell number,cell dead and reactive oxygen species(ROS)level of MS-1 cells after treatment with different magnetic field parameter.At the animal level,we analyzed the changes of microvascular in different mouse models which were exposed to static magnetic fields to initially explore the effects of static magnetic fields on angiogenesis in mouse models.The results showed that when MS-1 cells were exposed to the medium intensity static magnetic fields(0.4 T,0.18 T,0.08 T)with different directions for different time(3 h,6 h,18 h and 24 h),we found that the adhesion of MS-1 cells could be affected by the medium intensity static magnetic fields and the effect of the magnetic field was related to the exposure time and direction of the magnetic field.Moreover,the levels of ROS were reduced by 0.4 T and 0.18 T magnetic fields with different magnetic directions for 24 h.When palmitic acid(PA)caused moderate damage to MS-1 cells,exposure to 0.18 T downward magnetic field for 24 h reduced cell death,and exposure to 0.4 T downward magnetic field for 24 h increased the total number of cells.When MS-1 cells were seriously damaged by PA,the application of 0.08 T(downward)and 0.4 T(upward)static magnetic fields treatment for 24 h could reduce the ROS level,but the medium intensity static magnetic fields had no effect on the total cell number and cell death.These results suggested that medium intensity magnetic fields with a certain parameter could reduce the damage of palmitic acid to MS-1 cells and different cell states may influence the effect of static magnetic fields on cells.MS-1 cells cultured in the high glucose medium(50m M glucose)were exposed to 9.4 T upward high static magnetic field,the results showed that exposure to 9.4 T static magnetic field reduced the total number of cells,cell death,the ROS level and antioxidant capacity.In addition,microvascular count of tumor tissue was decreased by the 9.4 T magnetic field with different magnetic field directions,suggesting that exposure to9.4 T strong static magnetic field may inhibit tumor angiogenesis of lung cancer tissue.At present,our findings are preliminary,but it is of great significance for us to understand the effect and potential application of magnetic fields on angiogenesis.These findings also offer some reference significance for subsequent experimental exploration.At the same time,the results provide an experimental basis for us to use magnetic fields reasonably and safely at the same time,and may lay a foundation for angiogenesis therapy and other potential medical applications of magnetic fields in the future.