Experimental Study On Electromagnetic Radiation Parameters Screening For Vestibular Vertigo In Mice

With the popularization of various household appliances and the development of communication technology in modern society,the amount of electromagnetic radiation to the public is increasing.The harm of electromagnetic radiation to human beings and natural creatures has aroused wide concern of the society.A great deal of research has been done on the biological effects of electromagnetic radiation at home and abroad,and it has been found that the biological effects are mainly divided into thermal effects and non-thermal effects.The non-thermal effects of electromagnetic radiation will affect the nervous system,blood system,endocrine system and vestibular system of living things.With the increasing reports of balance disorders in people near electromagnetic fields,vestibular vertigo caused by non-thermal effects of electromagnetic radiation needs further study.The purpose of this paper is to use electromagnetic irradiation source that independently research and develop in laboratory on mice.Then we choose quantitative evaluation of vestibular vertigo.Additionally,we screen out the range of the dizziness-inducing parameters of the electromagnetic radiation source step by step by setting different experimental conditions of electrical stimulation and mechanical stimulation.This can provide some guidance to avoid to produce vestibular vertigo for who work in the existence of electromagnetic irradiation environment.The main work of this paper includes:1)In order to conduct electromagnetic radiation on experimental mice,the laboratory independently developed electromagnetic radiation sources.Firstly,the electromagnetic parameters of the radiation source are introduced,such as carrier frequency,pulse frequency,duty ratio etc.The main work of this paper will focus on screening out these electromagnetic parameters that can cause dizziness in mice.Then the radiation intensity of the electromagnetic radiation source is measured and compared with the international safety limit standard.Finally the specific absorption rate(SAR)of electromagnetic radiation absorbed by mice was estimated,which determines the electromagnetic radiation is given priority to with non-thermal effect.And this parameter will lay an important foundation for the following three detection methods of screening out electromagnetic parameters: behavioral experiment,videonystagmography experiment and electrophysiological experiment.2)In the behavioral detection method,aiming at the problem that the original fixator would cause excessive stress response in the experiment of mice,a kind of mouse flexible fixator was designed to improve the experimental efficiency.A rotating-stick experiment with quantifiable results was designed to preliminarily obtain the range of the dizziness-inducing parameter by comparing the residence time of different experimental groups.3)In the videonystagmography detection method,firstly we analyzes the common eye pupil identification method and the basis of mathematical morphology.Then the Starburst pupil localization algorithm based on the mice eyes was used on nystagmus video image processing to get nystagmus signal characteristics in mice.Finally,by comparing two methods we found that the rapidity and accuracy of the Starburst pupil localization algorithm is better and it can meet the requirements of the experiment;Subsequently,the nystagmus videos of mice were collected by using the nystagmus experimental platform.On the basis of the behavior detection method,the dizziness-inducing parameters were further screened out by comparing and analyzing the slow phase velocity(SPV)of nystagmus in different experimental groups.4)In the electrophysiological detection method,the physiological significance,waveform characteristics and handling methods of vestibular evoked potential(Vs EP)were firstly introduced.Secondly,the vestibular evoked potential(Vs EP)of the mice was collected by using the invasive subcutaneous electrode implantation method.On the basis of the videonystagmography detection method,the dizziness-inducing parameter was verified by comparing and analyzing the characteristics of the signal waveforms of different experimental groups.