| Garden waste is a general term for fallen leaves,lawn mowing grass,dead branches and other materials.It is a renewable resource.With the gradual increase of the base of garden waste,the disposal of garden waste needs to be solved urgently.This study in order to obtain from the strains with high efficient degradation of garden waste in plateau area,and study their degradation ability,using sodium carboxymethyl cellulose(CMC-Na)hydrolysis Congo red method,aniline blue transparent circle medium fade screen at the beginning of enzyme method,comparison method,in order to garden waste decomposition leaf as materials,comparing different strains of garden waste litter leaf of neutral detergent fiber(NDF),acid detergent fiber(ADF)degradation rate.PDA liquid flask was used to optimize the conditions for enzyme production,pot experiment was conducted to further determine the effect of compost products on plant growth,and a number of physiological and biochemical experiments were designed to explore the characteristics of strains.The research conclusions of this paper are as follows:(1)A total of 189 strains were isolated from cow dung,humus and other materials random Ly collected from the farm of Xizang Agricultural and Animal Husbandry University.Through plate identification,a total of45 strains with lignin or cellulose degrading ability were obtained.(2)The strains obtained from preliminary screening were inoculated into liquid medium for 7 days,and the enzyme activity of cellulase,filter paper enzyme,lignin peroxidase,manganese oxidase and laccase was determined by supernatant solution,respectively.Then the strains with higher enzyme activity were selected as the subsequent experimental strains.The results showed that among the 45 strains,F4-2 had the highest cellulase activity,which was 17.01 U/m L.The filter paper enzyme activity of F7-3 was the highest,which was 19.98 U/m L.The activity of lignin catalase in F6-4 was the highest,which was 95.57 U/m L.The manganese peroxidase activity of F6-4 was the highest,which was 67.1 U/m L.The laccase activity of the brown folded umbrella was the highest,which was45.44 U/m L.(3)The results showed that the contents of acid detergent fiber and neutral detergent fiber in the matrix after solid-state fermentation were lower than those in the control(CK),the degradation rate of neutral detergent fiber of strain F1-5 was the highest,which was 34.52%,and the content of neutral detergent fiber decreased by 25.35% compared with CK.The acid detergent fiber of strain F4-2 was the highest,which was34.26%,and the content of acid detergent fiber was 24.06% lower than that of CK.(4)The fermentation conditions of F1-5,F4-2,Maotouricola and Oytus orensis were optimized by changing the growth temperature,p H,inoculation amount,C/N ratio,different nitrogen sources and particle size of substrates using PDA liquid medium as the basic medium.The results showed that the strain had higher enzyme-producing activity under the conditions of p H 7,25? and C/N ratio of 25:1.(5)According to the degradation rate of the strains,the best degradation effects of the strains F1-5,F4-2,Maotouguican,Pleurococcus ostreus were selected as fermentation strains,solid-state fermentation was carried out again,and pot experiment was conducted to verify the effect of their composting products.The results showed that the compost product of the experimental group adding 2 strains of bacteria,when its added amount was 20%,promoted the plant growth obviously;In the experimental group with the addition of 4 strains of compost products,when the addition amount was 60%,the rooting promotion effect was obvious.When the addition amount was 30%,the chlorophyll content of the plant was the highest,which was 40.9 SPAD;Bacterial composting can promote the growth of plant stems and leaves,and can also effectively improve the water content of plants,while fungi can promote the growth of plant roots and the production of chlorophyll. |
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