Studying On The Enzyme Activity Of 10 Strains Of Cellulose Degrading Bacteria

With the continuous development of society,environmental problems are becoming increasingly prominent.Straw as a by-product of crops,yield and cellulose content is high,treatment straw plagued the growers and local environmental protection departments.It is low nutritional value and effective utilization rate and poor palatability when directly used to feed ruminants,such as cattle and sheep.Before spring plowing,it is burned to treat the polluted environment.Cellulose degrading bacteria,as a kind of microorganism group which can use cellulose efficiently,can not only achieve zero pollution to the environment,but also improve the nutritive value of coarse fodder.In this paper,the 10 strains of cellulose enzyme producing activity was studied,and make the formulation bacterias which could effectively degrade the straw cellulose.In this study,the research materials come from the soil and decomposed corn straw in the east and west of inner Mongolia such as,Tongliao,Hohhot,Bayannaoer,that with the depth of 4cm removed from the surface layer were packed in a self-sealing bag,squeezed out excess air and Storaged a 4-degree refrigerator in April 2016.The samble were separated,screened,purified,identified,antagonistic assay,bacterial system construction and fermentation characteristics were studied with combining the traditional method and modern molecular biology technology diversity analysis.And analyzed the kinds of main cellulose-degrading bacteria,the effects of the metal ions and surfactants on the bacterial system in inner Mongolia,and revealed the important role of cellulose-degrading bacteria in micro-storage feed with corn stalk as raw material.Under the condition of 30 centigrade culture,10 strains of Cellulase Producing Bacteria can be obtained by continuous culture of microcrystalline cellulose and sodium carboxymethyl cellulose as the sole carbon source and the sole nitrogen source of urea as the sole nitrogen source.By morphological observation of bacteria and the rescreening of Congo red identification medium,the bacteria that can stabilize growth of cellulase are obtained.Through mixed liquid culture and diversity analysis of microbial inoculum,the ratio of bacteria inside was 4:2:1,and they came from the same kingdom,three phylum,four order and nine genus respectively.The growth characteristics of cellulose bacteria,and study the optimum nitrogen source and ratio and temperature,pH,and the effects of metal ions and surfactants on the activity of cellulose are determined in this study.The results showed that optimum nitrogen source is the best peptone,the ratio of carbon and nitrogen is 10:1,fermentation temperature is 30?,pH is acidic,and the enzyme activity is the highest.The results of enzyme production showed that different concentrations of metal ions and surfactants could promote the yield of reducing sugar,among which Co²⁺and Cu²⁺are the best than the control group,the yield of reducing sugar increased by 2.2 times and 1.2 times when0.6 mmol/L Co²⁺and 1.5mmol/LCu²⁺were added,and the yield of reducing sugar increased by 70.2%when 6.8 mmol/L SDS was added.The experiments of solid fermentation of straw powder showed that the protein content of the fermentation products increased by 7%than natural fermentation when the ratio of Weissella confusa strainto Pediococcus pentosaceus strain is 2:1,the Cellulose fungus is 5%andhumidity is 65%.The research about screening of cellulose-degrading bacteria,the preparation of microbial inoculum,the optimal nitrogen source and carbon-nitrogen ratio,temperature,pH,metal ions and surfactants of the Microbial agent producing enzyme cellulase activity,and solid fermentation test,which showed that it laid a foundation for further improving the development of straw degradation bacteria agent and the effective utilization of crop straw.