Effects Of Tannin On Spatial Learning And Memory In Kunming Mice (Mus Musculus)

Spatial learning and memory is one of the important behaviors of animals in the process of adaptive evolution,which can affect animals' behavioral decision-making.Efficient learning and memory ability can reduce the cost of animals in survival competition,so that animals can quickly adjust their behavior according to memory,and adapt to the complex and changeable environment.Tannin(TA)is the most common plant secondary metabolite in nature.It is a defensive substance produced by plants in the process of evolution to protect themselves.Due to its chemical structure,tannin has antioxidant properties,which can intercept active free radicals and protect DNA from oxidative damage.It has antimutagenesis and anti-cancer properties.In addition,tannin can also combine with bacterial cell membrane and play a bactericidal role.Tannin can interact with macromolecular,precipitation macromolecules,such as form complex with protein,polysaccharide,so the intake of high levels of tannin will affect the digestion and absorption of the body,endogenous nitrogen loss,resulting in a decline in the bodyweight and organ toxicity.Therefore,it is significant to study the behavioral and physiological effects of tannins.At present,the effect of tannin on animals involves many aspects,but there are few studies on the effect of tannin on spatial learning and memory of animals.In order to further explore this question,Kunming mice(Mus musculus)were selected as objects,and tannin(0%,1%,3%,6%,9%)was added into the diet of mice.By measuring the body weight,carcass weight,exercise ability,learning and memory ability,main organs(heart,liver,kidney,spleen)and BDNF and Trk B expression in hippocampus,the effects of tannin on spatial learning and memory were analyzed.The main results of this study are as follows:1.There were significant differences in the body weight of mice fed with different concentrations of tannin.The body weight growth rate of TA-1% group was higher than that of CON group,while the body weight growth rate of TA-6% and TA-9% groups was lower than that of control group,and TA-9% had a certain mortality rate to mice.2.The total movement distance of mice in the open field in the TA-1% group was larger,while the total movement distance of mice in the TA-6% group was smaller.Therefore,low concentration(TA-1%)can increase the movement ability of mice,while 6% had a certain inhibitory effect on the movement ability of mice.3.Adding low concentration of tannin(TA-1%)to the diet can increase the spatial learning and memory ability of mice to a certain extent,while high concentration of tannin(TA-6%)has no significant effect.Thus,a moderate and low concentration of monotherapy(TA-1% group)can increase the spatial recognition and memory in mice.4.The body weight and carcass weight of mice fed the diet with low concentration of tannin(TA-1%)were higher than those in CON group,but those with high concentration(TA-6%)were lower than those in control group.The fresh weight of the main organs(heart,liver,kidney)of mice decreased with the increase of tannin concentration.5.The expressions of BDNF and Trk B in mice fed with low concentration(TA-1%)tannin were increased.In conclusion,tannins in diet have some influence on the motor ability,weight,main organs,spatial recognition and memory ability,BDNF and Trk B expression of Kunming mice,and the low concentration of tannins has a positive effect on the spatial recognition and memory,exercise ability and weight growth rate of mice,while high concentration tannins have certain toxicity and negative effects.The research provides a reference for understanding the behavioral and physiological effects of tannins,and it is significant to understand the interaction between animals and plants,and to promote the development and application of tannins.