The Cross-protective Characterization Of HSV-2 By Specific Immune Response Induced By HSV-1 Attenuated Strain M3

Human herpes simplex virus is a family of DNA viruses with complex gene regulation,Human herpes simplex virus type 1(HSV-1)and human herpes simplex virus type 2(HSV-2)can cause serious and widespread epidemic diseases such as herpes labialis,genital herpes,and nervous system infections.Although acyclovir drugs have been widely used clinically,there is still a lack of effective vaccines to prevent viral infections.Recent epidemiological observations have shown that the seropositivity rates of HSV-1 and HSV-2 are increasing in different age groups,therefore,the development of HSV-1 and HSV-2 related vaccines based on their latent infection and asymptomatic shedding properties is of great public health importance.Based on the similarity between the two in terms of genetic evolution and pathogenic mechanism,combined with the broad prospects of live attenuated vaccines in vaccine development,we use the weak pathogenicity of our previous research and can induce a significant immune response to HSV-1 attenuation Strain M3 explores the characteristics of immune cross-reactions between HSV-1 and HSV-2 from the perspective of serology and clinical protection,with a view to providing direct data for the control and prevention of diseases caused by these two virusesUnder the above premises,we immunized mice with the attenuated HSV-I strain(M3).which can induce a significant immune response,and detected the specific immune response induced by neutralizing antibodies at 28 days after immunization.To further analyze the clinical cross-protection of the immune response induced by the attenuated strain of M3 to HSV-2,HSV-1 wild strain and the HSV-2 wild strain were used to attack and infect mice in different ways then Observe the pathogenic situation of M3 immunized mice against HSV-1/2 virus infectionFrom the experimental observation and comparative analysis,we found that mice immunized with the attenuated HSV-1 strain M3 cannot induce specific anti-HSV-2 neutralizing antibodies.Attacked with HSV-2 in different ways after 28 days of immunization,viral load in tissues increases significantly in these mice,which shows little difference compared with unvaccinated blank mice.However,these immunized mice showed no obvious abnormal clinical manifestations,and histopathological damage also showed only a slight inflammatory reaction.Unlike HSV-2 infection,mice immunized with the attenuated M3 strain showed consistent serological and clinical protection,which means it can induce the production of specific anti-HSV-1 neutralizing antibodies and shows obvious protective effect after being subjected to wild-type HSV-1 attack.This result suggests that the immune response induced by HSV-1 and HSV-2 infections,although unable to show crossover of neutralizing antibodies In addition,the antiviral immune mechanism in the body can be able to mitigate the impact of viral infections on the tissues of the body through as yet unknown mechanistic manifestations The cross-protective model of pathological injury.