Mechanism Of Regulating Oil Accumulation By ABA Signaling Pathway Mediated By Apricot PP2C37

Prunus sibirica L.is a unique ecological and economic woody plant in Asia.It is mainly distributed in the East and north of China.Apricot is rich in protein and fat,and its fat content is generally about 55%,and its oil yield is 45.8%,which is nearly three times of that of soybean,which is why most scholars regard apricot as an excellent material for oil metabolism test.As we all know,abscisic acid(ABA)is a very important plant hormone,which is closely related to many physiological reactions and biochemical functions in plants,such as dormancy of plant seeds,opening and closing of plant stomata,etc.,and it is also a very important hormone that plants rely on when they are fighting against adversity,which has high research value.Therefore,this study evaluated the effects of exogenous abscisic acid on oil content,fatty acid composition,biodiesel performance and lipid compounds.According to the data,the abscisic acid signaling pathway plays an important role in the regulation of oil accumulation during the ripening of apricot(Sask).However,we know little about how ABA affects the composition and content of fatty acids(FA)in apricot.Therefore,the effect of Exogenous ABA on lipid compounds is worth studying.In this study,the effect of ABA on lipid accumulation during ripening of apricot was tested,and the dynamic rule of lipid content and fatty acid composition was determined.The results showed that:(1)the three key periods(early(4waa),middle(6waa)and late(8waa)were used for comparative analysis between ABA treated samples and control samples.The results showed that in the mature stage 8 WAA(weeks after anthesis,In addition,VLCFAs(c > 20)increased slightly,while palmitic acid(c16:0)and linolenic acid(C18:3)decreased slightly.The analysis shows that different FA biosynthesis is involved in the complex mechanism caused by ABA signal.(2)In this study,the data of lipomics showed that during the development of Sask,the treatment of Exogenous ABA resulted in the high accumulation of tag-18:1 / 18:1 / 18:2and tag-18:1 / 18:1 / 18:1.The results show that in Sask,in addition to PA derived DAG in Kennedy pathway,there are other ways to synthesize or modify DAG.By liposomal analysis,18:1 / 18:1 showed a similar time distribution between PC and DAG during the development of Sask,which means that the acyl chains between PC and DAG are closely linked.(3)In the study of the mechanism of ABA affecting the oil content of Siberian apricot,pp2c37,a protein phosphatase gene,which has a positive effect on ABA signal transduction,was screened out.The homologous genes of PP2C37 were screened,and the function of PP2C37 gene was further explored.The PP2C37 gene of Armeniaca sibirica was cloned by using the Armeniaca sibirica as the test material,and the function of the gene family was analyzed by genetic transformation.The mechanism of complex transcription and post transcriptional regulation of ABA signaling pathway existed in the accumulation process of Armeniaca sibirica was studied.(4)The homozygote of pp2c37 gene deletion mutant in Arabidopsis was screened and identified by "three primers" and semi quantitative PCR.The seed germination rate,root length and other phenotypes of pp2c37 deletion mutant and wild type Arabidopsis were observed.The above physiological experiments showed that under different concentrations of ABA(0,1,3,5 μ m)under stress,when the ABA concentration was greater than 3 μ m,the mutant showed higher sensitivity to exogenous ABA than wild type Arabidopsis.The effects of ABA on fat accumulation and properties of biodiesel were studied.Exogenous ABA increased the total oil content of apricot,but also affected the change of fatty acid composition.It is further proved that pp2c37 gene acts on Arabidopsis ABA stress response signal transduction pathway,activating stress-related transcription factors,which will help to improve the quality of apricot oil.