Gene Cloning, Vector Constructing, Prokaryotic Expressing Of Fatty Acid Elongase 1 Of Brassica Napus L. And Arabidopsis Transgenic Research

Widely existing in plants, fatty acids anabolism pathways is one of the most important metabolisms of plants.Plant oil is also a kind of important agricultural products. Now thousands of fatty acids have been discovered in plant oil.In recent decades, people have made great achievements in modifying fatty acid components in plant oil. Through classic breeding techniques, such as inducing mutation, screening positives, hybridizing, we obtained many crop strains with highly practical value and good agricultural qualities. In lately years, as the development of molecular biology and gene engineering, many genes whose translating products take part in the fatty acid synthesis were isolated and identified in oil plants especially rape plant. These results do accelerate the speed of improving rapeseeds oil composition.Erucic acid(C22: 1, â–³13) is a very long chain fatty acid(VLCFA).Wild rape plants are usually rich in erucic acid(normally 45%-60% in oil). In plant, erucic acid is synthesized under the catalysis of Fatty Acid Elongase 1, which locates in endoplasmic reticulum. The genetics study of the Arabidopsis thaliana VLCFAs mutants suggests that FAE1 regulates the elongation process of fatty acid chain above 18 carbon atoms and rate-limited steps of the anabolism of 18 : 1, 20 :1 and 18 : 0. So controling the amount of erucic acid could be achieved by regulating the expression the critical carbon-chain elongation gene.