Synthesis of recombinant antibody fragments for pesticide residue analysis using enzyme-linked immunosorbent assays

Posted on:2000-01-23

Degree:M.Sc

Type:Thesis

University:University of Guelph (Canada)

Candidate:Yau, Kerrm Yat Fai

Full Text:PDF

GTID:2463390014961552

Subject:Environmental Sciences

Abstract/Summary:

Antibody genes from a picloram-specific hybridoma were cloned, sequenced and characterized. Recombinant Fab fragments were expressed and secreted into the periplasmic space of recombinant Escherichia coli. Crude Fab preparation from the bacteria exhibited binding activity to picloram with an IC...

Keywords/Search Tags:

Recombinant

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