| Spodoptera litura is one of the serious pests around the world, attacking crop and greening plant. Owing to a great quantity of chemical insecticide been used for a long time, Spodoptera litura has resistance to organochlorine pesticide, organophosphorus pesticide, carbamic acid ester pesticide, pyrethroid insecticides. At the sametime, chemical pesticide polluted the environment. Fish and shellfish, even human, was hurt by it. Baculovirus has host specify, and it has no effect on nontarget animals. However, it has some disadvantages. For example, low speed of killing and narrow insecticidal spectrum. In order to solve the above problems one of the most popular approach is to improve the insecticidal properties of SPltMNPV by means of genetie engineering and moleeular biology.In this research, SpltMNPVⅡwas reformed. The main conclusions are as follows:1. Cloning and analysis of ctx-like gene, coming from AcMNPV.2. Recombinant baculovirus transfer vectors psk-Δegt-Pph–egfp and psk-Δegt- Pph-ctx-Pp10-egfp had been constructed successfully.3. A recombinant baculovirus SpltMNPV-Δegt-Pph-egfp was gained after cotransfection and repeatedly screen.4. Biological assay was been done about the recombinant baculovirus SpltMNPV-Δegt-Pph-egfp. It reduced the LT50 effectively.The results lay the foundation for the improvement and development of this virus insecticide. |
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