| For cellular biology, the idea of incorporating the capabilities of sample preparation, transport, reaction and analysis on a single chip has recently been revisited due to the development of a new generation of microfabrication tools. The rapid growth of interest in the microfabrication of lab-on-a-chip devices is fueled both by academia and industry because of the potential impact in fields such as biomedical, chemistry and biophysics. Direct photoablation with lasers has advantages of rapid prototyping, custom production and design flexibility.; In this thesis, the microfabrication of lab-on-a-chip structures for biophotonics application using F2 laser are presented. Fabrication of buried and surface waveguides provides a means of probing bio-materials. Investigation of F2-laser welding allows potential fusing for enclosure glass biochips. A combination of laser micromachining and refractive index profiling enable single-step integration of optical waveguides with microfluidic functions for optical sensing of microspheres which lays the groundwork for microfabrication of biochips. |
