| In human beings the circadian rhythm plays a role in maintaining sleep and wake cycles. The circadian rhythm is also involved in controlling many hormonal processes including cortisol, melatonin as well as others. The sleep and wake cycle of patients may be disrupted upon exposure to anesthesia during surgery. Disruption of the circadian cycle may result in increased recovery times due to prolonged wound healing, alteration in DNA repair mechanism, and disturbances in homeostasis. Drosophila melanogaster provides a reliable model to study time of day effects on circadian rhythms after exposure to sevoflurane. The aim of this study was to explore if there was a difference in A.M. and P.M. sleep and wake up times, after Drosophila was exposed to sevoflurane. The study utilized wild type, eyeless and ebony strains of Drosophila. These strains were exposed to sevoflurane and various emergence responses were examined. Four consecutive days of observational experiments were completed with sevoflurane exposure, during A.M. and P.M. on all three strains, to investigate differences in time of onset or emergence from anesthesia. The null hypothesis (H 0) for this research was that the time of day has no significant effect on the time of onset or emergence from anesthesia after 2% sevoflurane exposure. The statistical analysis in this study revealed that wild type flies recovered faster after 30 minutes of 2% sevoflurane during P.M. exposure as compare to A.M. exposure.;Key Words: Drosophila melanogaster, volitale/inhaled anesthetics, sevoflurane, circadian rhythm, bilological clock, wild type, eyeless, ebony, Pax6... |
