| Molecular genetics can give information about molecules that are indisputably involved inthe anesthetic response, and the basic approach is to identify genes that code for molecules thatdirectly control an animal's response to volatile anesthetics. Since there will undoubtedly be somevariation between organisms in the response to anesthetics, lines of organisms with divergentresponse to various anesthetics can be produced by selective breeding of those animals that displayextreme responses, and it is by comparing the results between these different systems that we arelikely to understand the global mechanisms by which volatile anesthetics function. To lucubrate the molecular targets of volatile anesthetics(sevoflurane), the suppressionsubtractive hybridization (SSH) was applied to compare mRNA levels from three different strainsof Drosophila melanogaster , the sevoflurane-sensitive strain (S) , the sevoflurane-resistant strain(R) , and the wild type strain (H) .And finally we got four different classes of relative genessensitive to volatile anesthetics in Drosophila melanogaster. (1)The first class of sensitive genes weidentified was related to lipid metabolism, such as CG33178, CG31689, Yolk protein, Osbp; (2)Thesecond identified class of sensitive genes was related to mitochondrial function. Such as Arf102F,Glutamine synthetase 2,Gs2, CG3705. (3)The third identified class of sensitive genes was relatedto neurotransmission. Such as CG9090, CG3597, CG11661, CG3609, CG7181, CG3967. (4)Thefourth identified class of sensitive genes was related to signaltransduction. Such as CG7766,CG5789, aPKC. The molecular functions of identified sensitive genes in our experiment still remainedunknown, but many of them were quite identical with the results of previous studies. It was surethat those selected genes may play potential role in the general anesthesia, and according to ourresults , it was presumed that multiple targets or mechanisms are involved in the action of generalanesthetics.
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