| Genetically modified crops have been widely adopted in agriculture. The most prevalent GM crops are tolerant to the herbicide glyphosate. Current safety evaluation methods for GM crops, based on substantial equivalence, do not screen for unintended changes which may occur in GM plants. The present study used subtraction suppression hybridization to screen for differences in gene expression between GM and non-GM, as well as between sprayed glyphosate and non-sprayed glyphosate soybeans, to evaluate substantial equivalence and to determine if SSH could be used to identify sequences used to genetically engineer plants. The putative function of differentially expressed transcripts identified related to carbon-dependent pathways and plant-stress response. While SSH did isolate the transgenic cp4 epsps sequence, limitations of the method make it inefficient for unknown transgene discovery. |
