| Here we report the partial cloning of a novel putative ribosomal protein S3 (rpS3) from dark-adapted octopus retinal cells. RpS3 is a component of the ribosomal small subunit, and it may be involved in a DNA repair mechanism. In this paper, RT-PCR was performed in order to amplify a part of rpS3 cDNA from dark-adapted octopus retinal cells. After RT-PCR products were separated by agarose gel electrophoresis, they were gel-purified and TA-cloned. Further M13 forward and reverse PCR analysis of resultant transformants confirmed the presence of approximate 500 by RT-PCT insert. The BLAST search program shows that the 453 nucleotide sequence and deduced 150 amino acid sequence are highly related to homology of rpS3, and this amino acid sequence contains K homology RNA-binding (KH) and truncated ribosomal protein S3, C-terminal domains. Both domains are highly conserved with a number of rpS3 sequences. |
