The Elementary Study On Effect And Menchansim Of Human Ribosomal Protein L6 Gene On The Drug Resistance In Leukemic Cells

Leukemia is one of the most common worldwide diseases. At present, chemotherapy is still the mayor method for leukemia. However, despite the high efficacy of the compound, the ability of cancer cells to become resistant to the drug remains a significant impediment to successful chemotherapy. Especially, multidrug resistance (MDR) of leukemic cells is responsible for the therapy failure and majority of deaths. Research of MDR and reversal of drug resistance have become urgently solved issue in leukemic therapy. Intensive efforts have been made through biochemical characterization, cellular, and genetic approaches to determine the basis of resistance and define genes that are involved in acquisition of drug resistance. To overcome the drug resistance, understanding of the mechanisms to obtain the resistance is necessary. The mechanisms of MDR involve many respects, such as overexpression of drug transporter protein, enhancement of detoxification enzymes and DNA repair enzymes, reduction of DNA topoisomerase, and decreased ability to initiate apoptosis, etc. All the known alteration, however, are not enough to explain fully the phenomenon of MDR in clinic. It have been report that many patients have acquired resistance to chemotherapy without undergoing these changes, suggesting that other unknown mechanisms could be involved.It is reported that approximately over 80 ribosomal proteins(RP) are found with in the large and small subunits of eukaryotic ribosomes. Although the ribosomal proteins are considered to mainly act as the place of protein synthesis, they have complex functions independent of their involvement in protein biosynthesis, which are called extraribosomal functions, and most of them have not been elucidated one by one, the precise functional role of each ribosomal protein is largely unknown. Increase, decrease and mutation of ribosomal proteins have been found in most human tumors. It is becoming increasingly clear that a growing number of ribosomal proteins exhibit various 'secondary' functions in cell proliferation and apoptosis, replication, transcription, RNA processing, DNA repair, autogenous regulation of translation, regulation of...