Hemicellulose and vitamin-C reduce xenobiotic-induced human T-cell alpha5beta1 integrin-mediated adhesion to fibronectin

Many studies have questioned the safety in using of household pesticides. Inflammation is one of the primary responses of our body to the potential exposure to pesticides. In vitro and in vivo studies have demonstrated that nutrient mixture supplementation reduces inflammation and expression of inflammatory markers associated with xenobiotic-induced T-cell adhesion mechanisms. Here, I investigated the effects of the nutrient mixtures, hemicellulose and vitamin-C, on xenobiotic-induced alpha5beta1 integrin-mediated T-cell adhesion to fibronectin.;As bifenthrin was applied to CEM SS T-cells, it stimulated T-cell adhesion to fibronectin at concentrations between 1.0 and 100 muM with a maximal stimulation of 8.3-fold at 10 muM. In contrast, hemicellulose completely blocked 100 muM bifenthrin-induced adhesion and reduced the effect of 10 muM bifenthrin by 5-fold. Another nutritional supplement, vitamin-C also reduced the level of T-cell adhesion at both 10 muM and 100 muM bifenthrin by 1.5-fold. The combination of hemicellulose and vitamin-C resulted in a 6.3 and 7.5-fold inhibition at 10 muM and 100 muM bifenthrin respectively. Antibody blocking studies demonstrated that bifenthrin induced CEM SS adhesion to fibronectin is mediated through alpha5beta1 integrin.;These data demonstrate that xenobiotic-induced alpha5beta1 integrin mediated T-cell adhesion to fibronectin is blocked by nutritional supplementation with hemicellulose and vitamin-C. These data also suggest the possibility that inflammatory responses associated with exposure to pollutants can be mitigated by nutritional supplementation.