Study On The Mechanism Of Action Of Majie Babu Ointment Based On TSLP In The Treatment Of Allergic Rhinitis In Mice

purposeThe incidence of allergic rhinitis(Allergic rhinitis,AR)is increasing year by year with the deterioration of climate and environmental pollution.At present,the main method of western medicine to treat allergic rhinitis is glucocorticoid therapy.The effect is worthy of affirmation,but long-term use has obvious side effects and is prone to recurrence.The external treatment of traditional Chinese medicine has a long history in the treatment of respiratory diseases.The clinical practice test has stable curative effect,and the side effects are not obvious.The previous research of the research group showed that Maji Babu Ointment has a good immunomodulatory effect In the early stage of allergic rhinitis,nasal mucosal epithelial cells can secrete TSLP after contacting allergens,which can trigger a lower-level immune response by directly or indirectly regulating DC cells,Th2 cells,and B cells.The compatibility of Majun Babu Ointment,Ephedra,White Mustard Seed,etc.,can not only relieve the cold,but also expel phlegm and drink,and has a good therapeutic effect on immune disorders in mice with allergic rhinitis.Based on this,this experiment used the regulation effect of Ma Jie Babu Ointment on TSLP in mice with allergic rhinitis to study the mechanism of action of Ma Jie Babu Ointment in the treatment of allergic rhinitis.method1 Divide mice into four groups:blank group,model group,plaster group,and western medicine group,and model dust mites,and evaluate the model by mouse behavior score,nasal mucosa HE staining,and serum specific IgE;2 ELISA method was used to detect the content of IgE and histamine in the serum of each group of mice,to clarify the therapeutic effect of Maji Babu Ointment on AR mice sensitized by dust mites;3 ELISA method to detect the content of TSLP in the serum of each group of mice;IHC method to observe the amount of TSLP in the nasal mucosa of each group of mice;WB,qPCR method to detect the content of TSLP in the lung tissue of each group of mice to determine the hemp The effect of Babu Ointment on TSLP in serum,nasal mucosa and lung tissue of AR mice sensitized by dust mite;4 ELISA method was used to detect the levels of IL-4 and IL-12 in the serum of mice in each group to explore the effect of Ma Jie Babu Ointment on the serum IL-4 and IL-12 of dust mite-sensitized AR mice:result1 Successfully established a dust mite AR mouse model and perfected the method of modeling dust mite AR mice.Behavioral score of mice:5 points in the model group,reaching the allergy standard.HE staining of mouse nasal mucosa:Compared with the blank group,the mucosal epithelial cells of the model group are arranged disorderly,cilia fall off,submucosal tissues are obviously hyperemia and edema,a large number of eosinophils infiltrate,and glandular hyperplasia.Serum IgE:Compared with the blank group,the IgE content of the model group was significantly higher(P<0.01).2 Therapeutic effect of Majubabu ointment on AR mice:HE staining of mouse nasal mucosa:Compared with the model group,the plaster group and western medicine group have significantly less inflammatory cell infiltration in submucosal tissues,hyperemia,edema,and glandular hyperplasia.Significantly reduced.Compared with the western medicine group,the cartilage tissue structure is clear in the plaster group,the structure is intact,continuous and undamaged,the fibrous tissue proliferation of the vascular wall is significantly improved,and the inflammatory cell infiltration of the vascular wall is significantly reduced,while the western medicine group and the model group have discontinuous tissue contours.Disorder,the phenomenon of intermittent destruction of blood vessel walls.IgE and histamine:Compared with the model group,the plaster group and western medicine group had significantly lower IgE and histamine content(P<0.01).3 The effect of Majie Babu Ointment on TSLP:ELISA of serum TSLP:Compared with the blank group,the content of TSLP was significantly increased(P<0.01);compared with the model group,the content of TSLP was significantly reduced in the plaster group(P<0.01).IHC of TSLP in nasal mucosa:Compared with the blank group,the model group has significantly more TSLP positive reaction points,and the number of inflammatory cell infiltration increased significantly,vascular congestion,edema,and fibrous tissue hyperplasia of the vessel wall.Compared with the model group,the number of TSLP positive reaction points and inflammatory cell infiltration in the ointment and western medicine groups were significantly reduced.Compared with the plaster group,the western medicine group has disordered and intermittent cell structure arrangement,and no complete tissue outline is seen,while the plaster group has a complete and clear outline.WB of lung tissue TSLP:Compared with the blank group,the bands of the model group were widened and deepened,but the difference was not obvious.The bands of the plaster group and the western medicine group were obviously shallower and thinner than the model group.QPCR of TSLPmRNA in lung tissue:Compared with the blank group,P>0.05,there is no significant statistical difference.Compared with the model group,the ointment and western medicine group showed a significant decrease in TSLPmRNA content(P<0.01).4 The effect of Majie Babu Ointment on IL-4 and IL-12:Compared with the blank group,the content of IL-4 was significantly increased(P<0.01),and the content of IL-12 tended to decrease(P>0.05).Comparing the ointment and western medicine group with the model group,the content of IL-4 was significantly reduced(P<0.01),and the content of IL-12 tended to increase(P>0.05).in conclusionThe therapeutic effect of Ma Jie Babu Ointment on AR mice may be achieved by reducing the content of TSLP,thereby inhibiting Th2-type inflammatory response.