A Comparative Study Of Astragalus And Panax Notoginseng On IL-1, IL-10 And TNF-? In Rats With "damaged Blood Stasis Syndrome"

Objectives: This study selected the Qi-enhancing drug Astragalus and blood-exchanging medicine Sanqi to interfere with the rat model of blood stasis.According to the changes of three indexes IL-1,IL-10 and TNF-? at different time periods,the study explored the effects of Astragalus and Panax Sanqi promotes the difference in injury repair effects,and analyzes the repair mechanism to provide an experimental basis for the use of Qi-enhancing drugs or blood-activating drugs for injured blood stasis syndrome.At the same time,it provides new medication ideas for the clinical prevention and treatment of sports trauma and injury that persists after the injury.Methods:1.78 male SD rats were selected and randomly divided into 4 groups: blank group,saline group(model control group),Astragalus group,and Sanqi group.The last three groups were divided into 4 groups according to the four phases of 1D,3D,5D,and 7D,a total of 13 group(N = 6).2.Quantitative gravity shock was used to model SD rats except the blank group.After successful modeling,rats in different groups were administered with saline,Astragalus and Sanqi suspension.3.Observe the general condition of the rats regularly every day,and measure the swelling degree(perimeter,front and back diameter)of the injured limb.Take skeletal muscle samples from the corresponding rats on the 1st,3rd,5th,and 7th days after modeling,and use immunosorbent assay to detect The concentrations of IL-1,IL-10,TNF-? in skeletal muscle and pathological observation of muscle tissue.Results:1.Perimeter of injured limb: longitudinal comparison(based on blank group): 1D after modeling,all three groups increased significantly;3D after modeling,there was a very significant difference in the saline group(<1D group));5D,7D after modeling There were still significant differences in the saline group(<3D group)).Horizontal comparison: 1D after modeling,Astragalus group <Sanqi group <saline saline group(p <0.05);3D after modeling,Astragalus group <normal saline group(p <0.01),Sanqi group <normal saline group(p <0.05).There is no difference.2.IL-1 concentration in skeletal muscle: longitudinal comparison(based on the blank group): 1D after modeling,the concentration of saline group and Sanqi group increased significantly;3D after modeling,the saline group increased significantly(<1D Group),Sanqi group significantly increased(<1D group);5D after modeling,saline group,Sanqi group significantly increased(both <3D group);7D after modeling,normal saline group,Sanqi group were all There were no differences;in the Astragalus group,there were no differences in the relative comparison group at the same time.Horizontal comparison: There was no difference between the saline group and the Sanqi group in each phase;after the model was made,the difference was significant in the Astragalus group compared with the normal saline group at 1D,3D,and 5D.3.Skeletal muscle IL-10 concentration: longitudinal comparison(based on the blank group): 1D after modeling,the concentration of the saline group increased significantly,and the concentration of the Sanqi group increased significantly;3D,the saline group increased significantly after modeling(<1D group);5D and 7D after modeling,the difference between the normal saline group was not obvious;there was no significant difference.Horizontal comparison: 1D after modeling,Astragalus group <saline group(p <0.05);3D after modeling,Astragalus group <physiological saline group(p <0.01),Sanqi group <normal saline group(p <0.05);There was no significant difference.4.Skeletal muscle TNF-? concentration: longitudinal comparison(based on the blank group): the concentration of the saline group was significantly increased,and the concentration of the Sanqi group was significantly increased;after 3D,the concentration of the saline group was significantly increased(<1D Group);5D after modeling,the concentration of the saline group increased significantly(<3D group);7D after modeling,the concentration of the saline group was not significantly different from that of the blank group.Lateral comparison: 1D after modeling,Astragalus group <normal saline group(p <0.05),Sanqi group <normal saline group(p <0.05);7D after modeling,Astragalus group <normal saline group(p <0.05);There was no significant difference.5.Results of skeletal muscle tissue pathology: muscle bundles in group 1D were disturbed,muscle fibers were dissolved and necrotic,and tissue damage was obvious;muscle bundles in groups 3D and 5D were slightly disturbed,and muscle fibers were necrotic;muscle bundles in group 7D were clear and muscle fibers were neat.Muscle bundles in Panax notoginseng 1D group were disordered,and muscle fibers were dissolved and necrotic in large amount,compared with the normal saline group in the same period.The muscle bundles of Astragalus 1D group were clearer,muscle fibers were slightly degenerated and necrotic,and the muscle tissues of the other groups were basically normal.Conclusions:1.After the early intervention of Astragalus membranaceus and Sanqi,both can reduce the maximum of swelling circumference and front and back diameter of rats,accelerate the disappearance of swelling and promote the recovery of injury;Astragalus has a stronger intervention effect than Sanqi.2.Astragalus and Sanqi achieve the purpose of immunomodulation and reduce immune inflammatory response by strongly inhibiting the immune-mediated effects of TNF-? and IL-1;Astragalus has stronger anti-inflammatory effect than Sanqi.3.Through the comparison of the effects of Astragalus and Sanqi on the repair of injury,it was found that the effect of gastric Astragalus suspension was significantly better than that of astral suspension of Sanqi,suggesting that the use of qi supplementation for the treatment of injured blood stasis syndrome may have a good effect in the early stage.Compared with the use of pure blood circulation and blood stasis medicine,there may be higher clinical value and better clinical efficacy.4.In the treatment of damaged blood stasis syndrome,the use of Qi-enhancing drug Astragalus may be better than the blood-enhancing drug Sanqi.It shows that the basic theory of traditional Chinese medicine has a clear guiding role for clinical practice and scientific experiments.Only by "being upright and innovative" can we truly promote the science of traditional Chinese medicine.