| Purpose:By animal experiment to observe chronic renal failure(CRF)model rats' changes of serum creatinine(Scr),blood uric acid(UA),blood urea nitrogen(BUN)and blood ?2microglobulin(?2-MG),serum albumin(ALB),tumor necrosis factor-?(TNF-?),urate transporter 1(URAT1)in renal tissue and ATP-binding cassette transporter G2(ABCG2)in ileum tissue,and to analyze the effets of Jiang Dan decoction on CRF and the way of lowering uric acid.Material and method:50 male SPF grade SD rats were randomly divided into the blank group of 12 and the model group of 38.The animal models of chronic renal failure were prepared using an adenine suspension of 2.5% at a dose of 200mg/ day(kg·d)for 21 consecutive days.The successfully prepared animal models were randomly divided into model group,Niaoduqing group and enema group.Every day,the rats in the Niaoduqing group were given Niaoduqing solution by gavage at a dose of 15ml/(kg·d).The rats in the other three groups were given distilled water by gavage at a dose of 15ml/(kg·d).Once every other day,all rats were anesthetized by intraperitoneal injection of 10% chloral hydrate(15ml/kg).After 5 minutes,the rats in the enema group were given nitrogen-lowering decoction by enema at a dose of 13ml/(kg·d).The rats in the other three groups were given distilled water by enema at a dose of 13ml/(kg·d).The depth of enema was about 8cm.After enema,the rats were inverted for about 3 minutes so that the liquid was fully exposed to the intestinal wall.After 14 days,each rats was taken 5ml of blood from the abdominal aorta to detected the values of Scr,BUN,UA,?2-MG and ALB by biochemical method and the value of TNF-? by Elisa.The left kidney of each rat was cut longitudinally and the expression level of URAT1 protein in renal tissue was determined by semi-quantitative interpretation.The protein expression level of ABCG2 in 2cm of ileum tissue of each rat was detected by semi-quantitative interpretation.Statistical software SPSS20.0 was used for data analysis.The measurement data of normal distribution were expressed as `x±s(mean ±standard deviation).One-way anova was used to compare the data between the groups,and LSD method was used to compare the data of each group in pairs.P<0.05 was considered tobe statistically significant.Results:1.Building the results:After the completion of model preparation,the rats in the model group were significantly weaker than those in the blank group,and their blood creatinine was also significantly increased.The kidney of the model group was observed to be larger and whiter.Under the light microscope,it was found that the glomerular atrophy,abnormal structure,shape and size,renal tubules atrophy,inflammatory cells infiltrating the interstitium,and vascular wall thickening were observed in the rats of the model group.2.General observation results: General observation results: after treatment,the mental state,diet and body of rats in enema group and Niaoduqing group were improved.3.Comparison of serum index results:The mean values of Scr,BUN,UA and TNF-?presented blank group<enema group<Niaoduqing group<model group.Except that there was no statistical difference in Scr between the enema group and the uriduqing group(P>0.05),there were statistical differences between the groups(P<0.05).The mean values of ?2-MG presented blank group <enema group<Niaoduqing group<model group,exclude blank groups and compare other groups,only the enema group and the model group had statistical differences(P<0.05).The mean values of ALB presented model group <Niaoduqing group<enema group<blank group,and there was no significant difference between enema group and model group,enema group and Niaoduqing group,Niaoduqing group and model group(P>0.05).4.Results of semi-quantitative interpretation of URAT1 protein expression in renal tissue:The protein was more expressed in proximal convoluted tubules.The mean value of URAT1 in renal tissues of each group presented model group>Niaoduqing group>enema group>blank group.There were statistical differences in the comparison between each group(P<0.05).5.Results of semi-quantitative interpretation of ABCG2 protein expression in ileal tissue:The mean value of ABCG2 in ileum was presented as blank group>model group>enema group>Niaoduqing group,except blank group,pairwise comparison of other three groups showed no statistical difference(P > 0.05).Conclusion:1.Jiang Dan decoction can effectively remove the small and medium molecular toxins in the blood of rats with chronic renal failure,and has the effect of improving renal function.2.Jiang Dan decoction protects renal function by reducing inflammatory factor TNF-? to inhibit renal fibrosis in rats with chronic renal failure.3.Jiang Dan decoction had a good effect on the removal of uric acid in rats with chronic renal failure.It can reduce uric acid by improving kidney function and reducing the expression of URAT1 protein in renal tubules to enhance the intensity of renal uric acid excretion in rats with chronic renal failure so that it can play an independent role in lowering uric acid.4.Jiang Dan decoction has little effect on intestinal urate transporter ABCG2. |
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