Explore The Mechanism Of "Knee Tongkang" In The Treatment Of Knee Osteoarthritis In Terms Of Joint Morphology And Inflammatory Factors

Purpose:To explore the mechanism of "Xitongkang" in treating knee osteoarthritis in terms of joint morphology and inflammatory factors.Material and method:1.Use integrated pharmacology to predict the main drug components and the target of osteoarthritis,explore the core gene functions and core signal pathways of the prescription,and expound the molecular mechanism of the prescription to treat osteoarthritis.2.Randomly divide 40 healthy SD rats into a blank group?distilled water,1ml / 100 g / d?,a model group?distilled water,1ml / 100 g / d?,and a control group?Zhuanggu joint pills,1.2g /kg / d?,experimental group?Xitongkang,5g crude drug / kg / d?.Except for the blank group,the rest of the groups used the anterior cruciate ligament dissection method for knee osteoarthritis modeling.Four weeks after the administration,an experimental study was conducted on the KOA model of the rat.After the specimens were processed,HE staining,toluidine blue staining,and Masson staining were used to observe the changes of chondrocyte structure and morphology,and the cartilage Mankin 's score was evaluated.The changes of TNF-?,IL-1? and PGE2 in serum were observed by ELISA.Results:1.Using the integrated pharmacology platform to obtain the common targets of the drug diseases of Jitongkang and KOA are GNAS and PTGS2.2.Using the integrated pharmacology platform,we obtained 20 core gene functions and 20 core pathways for KOA in the treatment of Xitongkang.3.In the experimental group,the structure,arrangement,morphology,cell matrix and collagen of chondrocytes were improved in HE staining,toluidine blue staining,and Masson staining.The mankin's score of the experimental group was better than that of the model group and control group P <0.01.4.Compared with the model group,the levels of TNF-?,IL-1? and PGE2 in the serum of the experimental group were significantly lowered?P <0.01?.Compared with the control group,the serum levels of TNF-??P <0.01?and IL The levels of-1??P <0.05?and PGE2?P <0.01?were significantly lowered.Conclusion:1.Use the integrated pharmacology platform to obtain the common target of Xitongkang and KOA for drug diseases as GNAS and PTGS2.2.Xitongkang can improve the Mankin's score of articular cartilage in the KOA model of rats,slow the destruction of cartilage,and have a protective effect on articular cartilage.3.Xitongkang can reduce the levels of IL-1? and TNF-? in the serum of model rats and reduce the inflammation of knee joints4.Xitongkang can reduce the level of PGE2 in the serum of model rats and has analgesic effect.5.Xitongkang can improve the damage of articular cartilage in model rats,inhibit inflammation,and has analgesic effect,which may be related to the inhibition of the expression of IL-1?,TNF-?,PGE₂.