| Purpose:By observing the effect of dioscin in Dioscorea nipponia extract on the sciatic nerve PI3 K / AKT / m TOR signaling pathway in mice with painful diabetic peripheral neuropathy,it can provide a theoretical basis for the treatment of painful diabetic peripheral neuropathy with dioscin from dioscin.Materials and methods:In the experiment,83 SPF male C57 BL / 6 mice weighing20-25 g were divided into 5 groups,and 12 mice were randomly selected as the normal group.After PDPN modeling,they were randomly divided into a model group,a high-dose dioscin group,and a low-dioscin In the dose group and α-lipoic acid group,12 rats in each group.After feeding for 8 weeks on high-fat feed,it was induced by intraperitoneal injection of 1%streptozotocin(STZ)70 mg / kg(prepared with p H 4.2,0.1 mol / L citrate buffer)mice diabetes model.Those with a blood glucose greater than 16.7 mmol / L were included in the observation object,and they were fed with normal food for 6 weeks.The mechanical pain threshold of mice was measured,and a drop of more than 50% was successful in modeling.Each group was observed at 0W before treatment,2W,4W,6W,8W after administration as the time point to observe the general state and daily activities of the mice.The weight of the mice was measured by electronic balance,the blood glucose of the mice was measured by the tail vein,Von Frey The silk method was used to measure the mechanical pain threshold of mice,and the thermal tail test was used to measure the thermal pain threshold of mice.At 8weeks,Western blot was used to detect the expression levels of PI3 K,p-AKT,and m TOR in the sciatic nerve of mice.Results:1.The mice in the model group had poor general condition,decreased behavioral activity,and were in a state of mental weakness,weight loss,dry and dry hair,adhesion into ears,and curled back.The mice in each treatment group also showed the above symptoms,but the degree was lighter than the model group.The high-dose dioscin group and the alpha-lipoic acid group were generally in better condition.2.Compared with the normal group,the body weight of the model group and eachadministration group was significantly reduced(P <0.01).Compared with the model group at4 weeks,6 weeks,and 8 weeks,the weight of mice in each administration group increased(P<0.01).3.Compared with the normal group,the blood glucose of the model group and each administration group increased significantly(P <0.01).Compared with the model group at 2weeks,4 weeks,6 weeks,and 8 weeks,the blood glucose levels of mice in each administration group decreased(P <0.01).At 8 weeks,compared with the alpha-lipoic acid group,the high-dose dioscin group had a significant drop in blood glucose(P <0.05).4.Compared with the normal group,the mechanical pain threshold of mice in the model group and each administration group decreased significantly(P <0.01).Compared with the model group at 4 and 8 weeks,the mechanical pain threshold of mice in each administration group was increased(P <0.01).At 8 weeks,the mechanical pain threshold of mice in the high-dose dioscin group was significantly higher than that in the low-dose dioscin group(P<0.05).5.Compared with the normal group,the heat pain threshold of the model group and each administration group decreased significantly(P <0.01).At 4 weeks and 8 weeks,compared with the model group,the heat pain threshold of the mice in each administration group increased(P <0.01).At 8 weeks,Compared with the low-dose dioscin group,the heat pain threshold of mice in the high-dose dioscin group and alpha-lipoic acid group was significantly higher(P <0.01).6.At 8 weeks,the sciatic nerve tissue proteins PI3 K,p-AKT,and m TOR of mice in each group were compared.Compared with the normal group,the protein concentration in the model group decreased(P <0.01).Compared with the model group,the protein concentration of each administration group increased,the high-dose dioscin group increased significantly(P<0.01);the alpha-lipoic acid group slightly increased(P <0.05);the low-dose dioscin group PI3 K,m TOR Slightly increased(P <0.05),p-AKT showed an increasing trend but no statistical significance.Conclusion:Dioscin in Dioscorea nipponia extract can increase the pain threshold of PDPN mice andactivate the PI3 K / AKT / m TOR signaling pathway,and the high-dose dioscin group is better than the low-dose dioscin group and the alpha-lipoic acid group. |
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