Effect Of Dioscin From Dioscorea Nigra Extract On P38MAPK And ERK_1/2 Protein Expression Of PDPN Mice Sciatic Nerve

Purpose: To study the effects of different concentrations of dioscin on the expression of p38 mitogen-activated protein kinase?p38MAPK?and extracellular regulatory protein kinase(ERK_1/2)protein in sciatic nerve of painful diabetic peripheral neuropathy?PDPN?mice.Further explore the role of p38 MAPK and ERK_1/2 signaling pathways in painful diabetic peripheral neuropathy and the analgesic mechanism of Chinese medicine Dioscorea nipponica extract dioscin in PDPN mouse models.Materials and methods: 83 adult male C57BL/6 mice,12 hours of circulating light,free access to food and water,after 7 days of normal feeding adaptation,12 mice were randomly selected from them as a normal control group,fed with normal feed,and the remaining 71 mice were high-fat After 8 weeks of feed feeding,the mice were fasted for 12 hours and then intraperitoneally injected with 1% streptozotocin 70 mg/kg?prepared with 0.1mol/l citrate buffer at p H 4.2?for 2 consecutive days to induce mice After successful diabetes,feed them with normal feed for 6 weeks,and determine the modelling of PDPN mice by measuring the decrease of MWT value of mice.The modules were randomly divided into four groups,namely: PDPN model group?group B,n=12?,low-dose diosgenin group?group C,n=12?,high-dose diosgenin group?group D,n=12?),?-lipoic acid group?group E,n=12?.In each group,the threshold of mechanical foot reflex reflex?marked as MWT value?was measured by the Von Frey Test filament method at 0w before treatment,4w and 8w after treatment.Record as TFL value).Each group was observed at different time points before treatment 0w,after treatment 2w,4w,6w,8w,etc.: general conditions?including hair color,mental state,food intake,daily activities,etc.?,body weight,blood sugar,and were executed on the 8th weekend The sciatic nerves of each group were taken as spares.Western blot?WB?was used to detect the expression of p38 MAPK and ERK_1/2 protein in the sciatic nerves of each group of mice.Results:1.Compared with group A,the weight of group B,C,D,E decreased significantly at 0w before treatment,2w,4w,6w,8w after treatment?P <0.01?;Compared with group A,the blood glucose of the group B,C,D,E increased significantly at each time point?P <0.01?;Compared with the group B,the blood glucose of the groups C,D and E decreased at 2,4,6,and 8 weeks?P <0.01?.2.All experimental mice developed hyperalgesia after intraperitoneal injection of STZ.Compared with group A,the MWT value and TFL value at each time point in groups B,C,D,and E decreased?P <0.01?;Compared with group B,the MWT value and TFL value of Groups C,D and E increased at 4 and 8 weeks?P <0.01?.3.At 8 weeks after treatment,the levels of p38 MAPK and ERK_1/2 in groups B and C were significantly higher than those in group A?P <0.01?.Compared with group B,the protein expression of p38 MAPK and ERK_1/2 in groups D and E Significantly decreased?P <0.01?.Conclusions:1.p38 MAPK and ERK_1/2 signaling pathways are involved in the pathological mechanism of PDPN mice.2.Dioscin may improve the pain symptoms of diabetic peripheral neuropathy mice by inhibiting p38 MAPK and ERK_1/2 signaling pathways,and play an analgesic effect.