| Purpose: To study the therapeutic effect of Buzheng Migu granule on osteoporosis in ovariectomized rats.Material and method:60 female SD rats were selected and randomly divided into six groups.control group,model control group,medicine control group,high dose group of Buzheng Migu granule,medium dose group of Buzheng Migu granule,low dose group of Buzheng Migu granule.Except the normal group,all the other groups were bilateral ovary removed,control group and model control group were given normal saline intragastric perfusion.The control group of medicine were given 12ug/kg estradiol solution twice a day,The low,middle and high dose groups were given 5.56 g / kg,11 g / kg,22 g / kg Buzheng Migu solution twice a day.After 12 weeks of administration,experimental materials were taken to record the weight and uterine weight of rats,Measurement of bone mineral density,Bone mineral conten,Biomechanical properties,Serum Ca,Serum P;Determination of serum alkaline phosphatase(ALP),estradiol(E2)and osteocalcin(BGP)content by elisa method;Observation of bone histomorphology of femur slices under electron microscope.Results:1.By comparing with the control group,The body mass,serum alkaline phosphatase and osteocalcin content in model control group increased after 12 weeks,while bone mineral content and biomechanical properties,BMD,serum Ca,P,E2 were all decreased.2.The effect of high dose group on improving bone metabolism and biomechanical properties of ovariectomized rats was better than that of the other groups,and it does not increase with the increasing of concentration,Comprehensive analysis of its mechanism of action.It is speculated that the action mechanism of Buzheng Migu granule is that it promotes the production of an estrogen-like substance in the body of rats,thus,the formation of osteoclasts is reduced,bone mineral density is increased,and the occurrence of high-conversion osteoporosis is inhibited.3.Comparison between Buzheng Migu granule groups,bone mineral density,biomechanical properties and bone histomorphology in high dose group improved slightly compared with those in middle group,low group.Moreover,it is not dose-dependent and has some reference value for clinical preparation of Buzheng Migu granule sepsis.4.The bone histomorphology,BMD,bone mineral content,biomechanical properties and serum E2 content in the control group and the medicine control group were significantly higher than those in the model group.There was no significant difference in the biomechanical characteristics and serum ALP,BGP content of BMD,between the medicine control group and the control group.Conclusions:1.The intervention of Buzheng Migu Granule on ovariectomized rats with simulated postmenopausal osteoporosis has statistical significance.Buzheng Migu Granule can alleviate or improve the bone metabolism of ovariectomized rats to a certain extent,and has a certain therapeutic effect on osteoporosis.2.Compared with the other groups,the effect of high-dose group of tonifying dense bone on improving bone metabolism of ovariectomized rats was better than that of other groups,and it was not concentration-dependent,analysing its action mechanism comprehensively,It is suggested that the action mechanism of high dose group may be that it can promote the production of an estrogen-like substance in rats,thus promote bone formation,inhibit bone destruction,and then increase bone mineral density(BMD).Prevention of high-conversion osteoporosis.3.Estrogen has the ability to improve bone mineral density,biomechanical properties and bone morphology of ovariectomized rats,but its anti-osteoporosis effect is still inferior to the normal physiological effect of normal rats. |
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