| Objective:To investigate the effect of Huxinkang on hyperlipidemia in TLR4 k nockout mice and its effect on ?-catenin and GSK-3?.Methods:10 wild C57BL/10 wild mice were used as wild control group.Accor ding to the random number table,35 male TLR4 knockout mice were divided i nto 60 models of hyperlipidemia model and 10 rats of blank control group.Ora l feed was provided daily to the wild control group and the blank control grou p.The high-fat diet containing 21% fat and 0.15% cholesterol was supplied to t he high-fat model group daily to construct a hyperlipidemia model.On the 13 th week of feeding,4 mice were randomly selected from the high-fat model grou p,blood was collected by eyeball extraction,blood lipids were measured,and a significant increase in blood lipids was found to indicate successful modeling.Subsequently,the remaining 50 mice in the high-fat model group were randoml y divided into Wnt inhibitor group,model group,medium-dose Huxinkang grou p,high-dose Huxinkang group,and atorvastatin calcium tablets group.The mice in the group were 5 males and 5 females.At the 14 th week,the animals were given drug intervention.According to the equivalent surface area between hum an and mouse,the medium dose was twice as high as the human dose,the hig h dose was 4 times,and the atorvastatin calcium group was Equivalent dose.T he high-and medium-dose Huxinkang group and the atorvastatin calcium tablet group were administered at 5.0 g/(kg/d),2.5 g/(kg/d),and 1.25 mg/(kg/d),respe ctively.The above-mentioned drug was completely mashed and placed in distille d water to be stirred and dissolved sufficiently to prepare a suspension,0.5 ml once per administration,once a day.At the same time,wild control,blank grou p,Wnt inhibitor group distilled water 0.5ml gavage,once/d.Wnt inhibitor group was given Wnt inhibitor XAV939,2 ug,intraperitoneal injection,diluted with physiological saline,0.3 ml/d,once/d.Wild control,blank group,high and medi um dose Huxinkang group,atorvastatin calcium tablets were given normal saline,intraperitoneal injection,0.3ml/d,once/d.Each group was administered continuo usly for 4 weeks.At the end of the fourth week of continuous drug interventio n,the material was taken.The blood and aorta of each group of mice were taken.The blood lipids were measured by enzyme colorimetry,the content of ?-cat enin and GSK-3? protein was measured by ELISA,and the expression of ?-cat enin and GSK-3? genes was measured by real-time PCR.Results:1.Blood lipid content The levels of triglyceride,cholesterol,high-density lipo protein and low-density lipoprotein in the blood of the model group were signifi cantly higher than those in the normal group(P<0.05),while the content of the wild-type control group was slightly higher normal group.The increase in high-density lipoprotein is considered to be due to the stress response of the body a fter stimulation by the other three.Atorvastatin calcium tablets and Huxinkang c an reduce the content of triglyceride and cholesterol(P<0.05),while Wnt inhibit ors can lower the cholesterol content(P<0.05),but the effect of reducing triglyc eride is not obvious.Atorvastatin calcium tablets had the strongest effect on red ucing triglyceride and cholesterol(P<0.05).The effect of high-dose Huxinkang g roup was slightly higher than that of the medium-dose Huxinkang group.The th ree have relatively low effects on low-density lipoprotein and high-density lipopr otein.2.?-catenin,GSK-3? protein content The ?-catenin protein content in the mo del group was significantly higher than that in the normal group(P<0.01).The content of ?-catenin protein in the wild control group was slightly higher than t hat in the normal group.Compared with the model group,all of them could re duce the content of ?-catenin protein(P<0.05).The Wnt inhibitor group had stro nger ?-catenin protein content than the Huxinkang and atorvastatin calcium table ts;atorvastatin calcium tablets group.It is stronger than the Huxinkang Group.The GSK-3? protein content in the model group was significantly lower than th at in the normal group(P<0.01).The content of GSK-3? protein in the wild co ntrol group was slightly lower than that in the normal group.Compared with th e model group,all of them could increase the GSK-3? protein content,and the Wnt inhibitor group increased the GSK-3? protein content more strongly than t he Huxinkang and atorvastatin calcium tablets;atorvastatin calcium The film gro up is stronger than the Huxinkang group.3.?-catenin,GSK-3? gene expression The expression level of ?-catenin genein the model group was significantly higher than that in the normal group(P<0.01).Compared with the model group,all of them could inhibit the expression of ?-catenin gene,and the Wnt inhibitor group and the atorvastatin calcium ta blets group had stronger effects(P<0.01),and the Huxinkang group also had si gnificant inhibition(P<0.05);Wnt inhibitors inhibited the expression of ?-catenin gene most strongly,and its effect was greater than that of the middle dose of Huxinkang group(P<0.05).The atorvastatin calcium tablets group was slightly s tronger than the Huxinkang group.The expression of GSK-3? gene in the mode l group was significantly lower than that in the normal group(P<0.01).Compar ed with the model group,all of them could increase the expression level of GS K-3? gene,and the Wnt inhibitor group and the atorvastatin calcium tablet grou p had stronger effects(P<0.01),and the protective heart also had a significant i ncrease(P<0.05);Wnt inhibitors had the strongest expression of GSK-3? gene,and its effect was greater than that of the middle dose Huxinkang group(P<0.05).The atorvastatin calcium tablets group was slightly stronger than the Huxink ang group.Conclusion:1.Huxinkang can significantly reduce the content of triglyceride and choles terol in hyperlipidemia mice.2.The expression of ?-catenin protein and gene was significantly increased in hyperlipidemia,while GSK-3? was significantly decreased.3.Huxinkang can reduce the expression of ?-catenin protein and gene,and increase the expression of GSK-3? protein and gene.This means that the heart can achieve the role of prevention and treatment of atherosclerosis. |
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