| Objective:This experiment from the Angle of the reinforcement theory of TCM,acupotomology understanding of knee osteoarthritis,muscle,tendon mechanical behavior as the breakthrou-gh point,observe the needle knife therapy for KOA rabbit-the tibialis anterior muscle gastrocnemius methods of tibialis anterior muscle and tendon tensile,creep and stress relaxation,the influence of the loose solution designed to verify the needle knife through regulating the knee joint muscle,tendon system the principle of treatment of KOA,reveal-ing TCM needle knife"jin hong"bone method scientific connotation.Methods:28 healthy and clean 6-month-old New Zealand rabbits were randomly divided into blank group,model group,electroacupuncture group and acupotomology group.The modified Videman method was used to immobilize the left hind leg in straight position.After 1 week of successful modeling,the blank group and model group did not intervene,and the electroacupuncture group and acupotomology group did not intervene for 3 weeks each.In the electroacupuncture group,acupuncture was performed on the left hind limb"xuehai","liangqiu","inner knee eye" and "outer knee eye",3 times a week.In the acupotomology group,the insertion points of the femoral and lateral muscles and the inner and outer genicular eyes were used as the injection points for treatment,twice a week.Animals in each group were sampled and tested 1 week after the intervention.Test indicators include:(1)Surface electromyography of tibial anterior muscle and gastrocnemius muscle:Surface electromyography was used to measure the surface electromyography of the tibialis anterior muscle of the left hind limbs of KOA rabbits at 0g,500g and 1000g in vivo.(2)Biomechanical behavior test of tibial anterior muscle tendon:easure the length,width and thickness of the sample with a reading vernier caliper.The Bose Electro Force 3300 fatigue tester was fitted with a special anti-slip clamping device for stress relaxation,creep and tensile testing and data recording.The creep rate,stress relaxation rate,elastic modulus,maximum stress and maximum strain of the specimens were calculated by using the load displacement curve.Results:1.Surface emg of anterior tibial muscle:with the increase of the load of left anterior tibial muscle in KOA rabbits,the surface emg of left anterior tibial muscle in each group showed an upward trend.(1)the emg values of the surface of the left anterior tibial muscle in each group under no load showed that:compared with the blank group,the emg values of the surface of the left anterior tibial muscle in the model group were significantly decreased(P<0.01).Compared with the model group,the emg value of the surface integral of the left anterior tibial muscle in the acupotomy group was significantly increased(P<0.01),and the emg value of the surface integral of the left anterior tibial muscle in the electro-acupuncture group was significantly increased(P<0.01).Compared with the electroacu-puncture group,the emg value of the surface integral of the left tibial anterior muscle in the acupotomy group showed an upward trend,and there was no significant difference between the two groups(P>0.05).(2)the emg values of the surface of the left anterior tibial muscle of each group under 500g load showed that:compared with the blank group,the emg values of the surface of the left anterior tibial muscle of the model group were significantly decreased(P<0.01).Compared with the model group,the emg value of the surface integral of the left anterior tibial muscle in the acupotomy group was significantly increased(P<0.01),and the emg value of the surface integral of the left anterior tibial muscle in the electroacupuncture group was significantly increased(P<0.01).Compared with the electro-acupuncture group,the emg value of the surface integral of the left tibial anterior muscle in the acupotomy group increased significantly(P<0.01).(3)the emg values of the surface of the left anterior tibial muscle of each group under 1000g load showed that:compared with the blank group,the emg values of the surface of the left anterior tibial muscle of the model group were significantly decreased(P<0.01).Compared with the model group,the emg value of the surface integral of the left anterior tibial muscle in the acupotomy group was significantly increased(P<0.01),and the emg value of the surface integral of the left ante-rior tibial muscle in the electroacupuncture group was significantly increased(P<0.05).Compared with the electroacupuncture group,the emg value of the surface integral of the left tibial anterior muscle in the acupotomy group showed a downward trend,and there was no significant difference between the two groups(P>0.05).2.Surface emg value of gastrocnemius muscle:with the increase of left gastrocnemius muscle load in KOA rabbits,the surface emg value of left gastrocnemius muscle in each group showed an upward trend.(1)the results of emg values on the surface of left gastrocnemius muscle in each group under no load showed that:compared with the blank group,the emg values on the surface of left gastrocnemius muscle in the model group were significantly decreased(P<0.01).Compared with the model group,the emg value of the surface integral of the left gastrocnemius muscle in the acupotomology group was signifi-cantly increased(P<0.01),and the emg value of the surface integral of the left gastrocnemius muscle in the electroacupuncture group was significantly increased(P<0.05).Compared with the electroacupuncture group,the surface emg value of the left gastrocnemius muscle in the acupotomy group showed an upward trend,and there was no significant difference between the two groups(P>0.05).(2)the emg values on the surface of the left gastrocnemius muscle of each group under 500g load showed that:compared with the blank group,the emg values on the surface of the left gastrocnemius muscle of the model group were significantly decreased(P<0.01).Compared with the model group,the emg value of the surface integral of the left gastrocnemius muscle in the acupotomology group was significantly increased(P<0.05),and the emg value of the surface integral of the left gastrocnemius muscle in the electroacupuncture group was significantly increased(P<0.01).Compared with the electroacupuncture group,the surface emg value of the left gastrocnemius muscle in the acupotomy group showed a downward trend,and there was no significant difference between the two groups(P>0.05).(3)the emg values of left gastroc-nemius muscle surface score of each group under 1000g load showed that:compared with the blank group,the emg values of left gastrocnemius muscle surface score of model group were significantly decreased(P<0.01).Compared with the model group,the emg value of the surface integral of the left gastrocnemius muscle in the acupotomology group was significantly increased(P<0.01),and the emg value of the surface integral of the left gastro-cnemius muscle in the electroacupuncture group was significantly increased(P<0.01).Compared with the electroacupuncture group,the surface emg value of the left gastrocnemius muscle in the acupotomy group showed an upward trend,and there was no significant difference between the two groups(P>0.05).3.Creep rate and stress relaxation rate of the tibial anterior muscle tendon:(1)the creep rate of the left tibial anterior muscle tendon of each group showed that:compared with the blank group,the creep rate of the left tibial anterior muscle tendon of the model group was significantly increased(P<0.01).Compared with the model group,the creep rate of left tibial anterior muscle tendon in the acupotomy group decreased significantly(P<0.01),and that of the electroacupuncture group decreased significantly(P<0.01).Comp-ared with the electroacupuncture group,the creep rate of left tibial anterior muscle tendon in the acupotomy group showed an increasing trend,and there was no significant difference between the two groups(P>0.05).(2)the stress relaxation rate of the left tibial anterior muscle tendon of each group showed that:compared with the blank group,the stress relaxation rate of the left tibial anterior muscle tendon of the model group was significantly increased(P<0.01).Compared with the model group,the stress relaxation rate of the left tibial anterior muscle tendon in the acupotomy group decreased significantly(P<0.01),and the stress relaxation rate of the left tibial anterior muscle tendon in the electroacupuncture group decreased significantly(P<0.01).Compared with the electroacupuncture group,the stress relaxation rate of the left tibial anterior muscle tendon in the acupotomy group decreased significantly(P<0.01).4.Tibial anterior muscle tendon stretch(maximum stress,maximum strain,elastic modulus):(1)the maximum stress of left tibial anterior muscle tendon in each group showed that:compared with the blank group,the maximum stress of left tibial anterior muscle tendon in the model group significantly decreased(P<0.01).Compared with the model group,the maximum stress of the left tibial anterior muscle tendon in the acupotomy group showed an upward trend(P>0.05),and the maximum stress of the left tibial anterior muscle tendon in the electroacupuncture group showed an upward trend(P>0.05).Compared with the electroacupuncture group,the maximum stress of the left tibial anterior muscle tendon increased in the acupotomy group,and there was no significant difference between the two groups(P>0.05).(2)the maximum strain of the left tibial anterior muscle tendon in each group showed that:compared with the blank group,the maximum strain of the left tibial anterior muscle tendon in the model group increased significantly(P<0.01).Compared with the model group,the maximum strain of the left tibial anterior muscle tendon in the acupotomy group was significantly decreased(P<0.01),and the maximum strain of the left tibial anterior muscle tendon in the electroacupuncture group was significantly decreased(P<0.01).Compared with the electroacupuncture group,the maxim-um strain of the left tibial anterior muscle tendon in the acupotomy group showed an upward trend,and there was no significant difference between the two groups(P>0.05).(3)the elastic modulus of the left tibial anterior muscle tendon of each group showed that:compared with the blank group,the elastic modulus of the left tibial anterior muscle tendon of the model group significantly decreased(P<0.01).Compared with the model group,the elastic modulus of the left tibial anterior muscle tendon of the acupotomy group was significantly increased(P<0.01),and that of the electroacupuncture group was signifi-cantly increased(P<0.01).Compared with the electroacupuncture group,the elastic modu-lus of the left tibial anterior muscle tendon of the acupotomy group showed an upward trend,and there was no significant difference between the two groups(P>0.05).Conclusion:One of the mechanisms of acupotomology in the treatment of KO A may be to restore the mechanical balance state of the knee joint by adjusting the biomechanical behavior of the soft tissue around the knee joint,which reveals the scientific connotation of acupotomology in Chinese medicine. |
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