Study On The Response Characteristics Of External Friction Stimulation To The Protein Expression Of Fascial Fibroblasts And The Biomechanical Mechanism Of Massage "reducing Swelling And Relieving Pain"

Background:The rubbing manipulation used to treat periarthritis of shoulder,strain of lumbar muscles,tenosynovitis,and chronic arthritis of the lower back and chest and limbs,collectively referred to as chronic fascia injury,it has the efficacy of detumescence and analgesia.Chronic fascia injury is associated with aseptic inflammation,and the inflammatory lesions is associated with Inflammatory factor like cell factor and others,adjusting inflammatory cytokines maybe is the mechanism of rubbing manipulation.Objective:Study in vitro of friction force stimulation on Meridian points fascia connective tissue fibroblasts changes of protein expression.Revealing the massage rubbing manipulation method reduce inflammation cell biology mechanics mechanism of damage,to perfect massage therapy principle provide new theoretical basis.Methods:1、Execute 14 days pregnant SD female rats and get fetal rats GV near about 5mm wide subcutaneous tissue,take the processed Subcutaneous fascia connective tissue into disinfected culture dish,extract,cultivate and Amplificate cells.2、The experimental method of cell biomechanics,amplificated fibroblasts were randomly divided into blank control group,low intensity of friction group,moderate intensity of friction group and high intensity of friction group,through the horizontal shaking table friction load for different groups,the speed of the level of shaking table including:blank control group 0 R/min,low intensity of friction group 60 R/min,moderate intensity of friction group 120 R/min,high intensity of friction group 180 R/min.Force for 2 hours every day,continue 3 days to develop in the incubator for the last 2 hours after Forced,and put the cells into the frozen tube and put the frozen tube into liquid nitrogen tank.3、Using the antibody protein chip technology detect different groups of Meridian points fascia connective tissue fibroblasts.Results:1、3 groups of friction force both adjust the meridians local tissue fibroblasts Interleukin and its receptor,compared with blank group,28 proteins adjusted in low intensity of friction group,51 proteins adjusted in moderate intensity of friction group,3 8 proteins adjusted in high intensity of friction group.the compare of all adjustχ2=102.503,P=0<0.01.Between 3 groups of friction force,there were different adjust of Interleukin and its receptor,compared with blank group,2 proteins raised and 26 proteins lowed and 28 stable proteins in low intensity of friction group,1 protein raised and 50 proteins lowed and 5 stable proteins in moderate intensity of friction group,2 proteins raised and 36 proteins lowed and 18 stable proteins in high intensity of friction group,the compare of each other adjust χ2=23.833,P=0<0.01.Most adjusted in moderate intensity of friction group and least adjusted in low intensity of friction group.2、3 groups of friction force both adjust the meridians local tissue fibroblasts Growth factor and its receptor,compared with blank group,31 proteins adjusted in low intensity of friction group,47 proteins adjusted in moderate intensity of friction group,35 proteins adjusted in high intensity of friction group.the compare of all adjustχ2 =107.142,P=0<0.01.Between 3 groups of friction force,there were different adjust of Growth factor and its receptor,compared with blank group,21 proteins raised and 10 proteins lowed and 36 stable proteins in low intensity of friction group,6 protein raised and 41 proteins lowed and 20 stable proteins in moderate intensity of friction group,14 proteins raised and 21 proteins lowed and 32 stable proteins in high intensity of friction group,the compare of each other adjust χ2=33.555,P=0<0.01.Most adjusted in moderate intensity of friction group and least adjusted in low intensity of friction group.3、3 groups of friction force both adjust the meridians local tissue fibroblasts Tumor necrosis factor and its receptor,compared with blank group,14 proteins adjusted in low intensity of friction group,22 proteins adjusted in moderate intensity of friction group,16 proteins adjusted in high intensity of friction group.the compare of all adjust x2 =36.734,P=0<0.01.Between 3 groups of friction force,there were no different adjust of Tumor necrosis factor and its receptor,compared with blank group,7 proteins raised and 7 proteins lowed and 18 stable proteins in low intensity of friction group,6 protein raised and 16 proteins lowed and 10 stable proteins in moderate intensity of friction group,7 proteins raised and 9 proteins lowed and 16 stable proteins in high intensity of friction group,the compare of each other adjust χ2=6.651,P=0.156>0.05.4、3 groups of friction force both adjust the meridians local tissue fibroblasts Chemokine and its receptors,compared with blank group,12 proteins adjusted in low intensity of friction group,26 proteins adjusted in moderate intensity of friction group,17 proteins adjusted in high intensity of friction group.the compare of all adjustχ2 =51.112,P=0<0.01.Between 3 groups of friction force,there were different adjust of Chemokine and its receptors,compared with blank group,10 proteins raised and 2 proteins lowed and 34 stable proteins in low intensity of friction group,6 protein raised and 20 proteins lowed and 20 stable proteins in moderate intensity of friction group,7 proteins raised and 10 proteins lowed and 29 stable proteins in high intensity.of friction group,the compare of each other adjust χ2=20.019,P=0<0.01.Most adjusted in moderate intensity of friction group and least adjusted in low intensity of friction group.5、3 groups of friction force both adjust the meridians local tissue fibroblasts Adhesion molecules,compared with blank group,11 proteins adjusted in low intensity of friction group,9 proteins adjusted in moderate intensity of friction group,9 proteins adjusted in high intensity of friction group.the compare of all adjust χ2 =23.834,P=0.001<0.05.Between 3 groups of friction force,there were no different adjust of Adhesion molecules,compared with blank group,6 proteins raised and 5 proteins lowed and 6 stable proteins in low intensity of friction group,1 protein raised and 8 proteins lowed and 8 stable proteins in moderate intensity of friction group,5 proteins raised and 4 proteins lowed and 8 stable proteins in high intensity of friction group,the compare of each other adjust χ2=5,393,P=0.249>0.05.6、3 groups of friction force both adjust the meridians local tissue fibroblasts Signaling pathway protein and Signaling pathways negative feedback regulating factor,compared with blank group,15 proteins adjusted in low intensity of friction group,15 proteins adjusted in moderate intensity of friction group,13 proteins adjusted in high intensity of friction group.the compare of all adjust χ2 =32.310,P=0<0.01.Between 3 groups of friction force,there were no different adjust of Signaling pathway protein and Signaling pathways negative feedback regulating factor,compared with blank group,6 proteins raised and 9 proteins lowed and 7 stable proteins in low intensity of friction group,2 protein raised and 13 proteins lowed and 7 stable proteins in moderate intensity of friction group,4 proteins raised and 9 proteins lowed and 9 stable proteins in high intensity of friction group,the compare of each other adjust χ2=3.380,P=0.496>0.05.7、3 groups of friction force both adjust the meridians local tissue fibroblasts Other proteins(specific),compared with blank group,14 proteins adjusted in low intensity of friction group,34 proteins adjusted in moderate intensity of friction group,22 proteins adjusted in high intensity of friction group.the compare of all adjustχ2 =72.478,P=0<0.01.Between 3 groups of friction force,there were different adjust of Other proteins(specific),compared with blank group,7 proteins raised and 7 proteins lowed and 35 stable proteins in low intensity of friction group,1 protein raised and 33 proteins lowed and 15 stable proteins in moderate intensity of friction group,3 proteins raised and 19 proteins lowed and 27 stable proteins in high intensity of friction group,the compare of each other adjust x2=30.207,P=0<0.01.Most adjusted in moderate intensity of friction group and least adjusted in low intensity of friction group.8、3 groups of friction force both adjust the meridians local tissue fibroblasts Other proteins(no specific),compared with blank group,2 proteins adjusted in low intensity of friction group,12 proteins adjusted in moderate intensity of friction group,6 proteins adjusted in high intensity of friction group.the compare of all adjustχ2 =27.778,P=0<0.01.Between 3 groups of friction force,there were different adjust of Other proteins(no specific),compared with blank group,1 proteins raised and 1 proteins lowed and 17 stable proteins in low intensity of friction group,0 protein raised and 12 proteins lowed and 7 stable proteins in moderate intensity of friction group,1 proteins raised and 5 proteins lowed and 13 stable proteins in high intensity of friction group,the compare of each other adjust χ2=15.441,P=0.004<0.05.Most adjusted in moderate intensity of friction group and least adjusted in low intensity of friction group.9、3 groups of friction force both adjust the meridian points fascia connective tissue fibroblasts,χ2 =337.325,P=0<0.01.Between 3 groups of friction force,there were different adjusted,χ2=68.784,P=0<0.01.Most adjusted in moderate intensity of friction group and least adjusted in low intensity of friction group,general law:moderate intensity>high intensity>low intensity.Conclusion:1、The meridians local tissue fibroblasts can synthesize a series of biochemical active substances with friction stimulation,and different adjusted different biochemical active changed.2、Most adjusted in moderate intensity of friction group and least adjusted in low intensity of friction group,and high intensity of friction group between the two,explain there was peak of friction stimulation.3、Because the cell physiological factor can enhance immune function and pathologically can cause inflammatory lesions,The reduced of Meridian points fasciaconnective tissue fibroblasts cytokine expression synthesis by friction to stimulate,tosome extent,it can be seen that the biomechanical mechanism of massage to relieve inflammation injury about treat chronic fascia injury.4、Through the experimental research to reveal the mechanism of friction stimulation,to perfect massage therapy principle provide new theoretical basis.