The Effect Of Different Concentrations Of Pomegranate Peel Polyphenol Ointment On Sebum, Linoleic Acid And Cholesterol

Objective:Objective to explore the effect of pomegranate peel polyphenol made from pomegranate peel after making ointment on sebum and its components linoleic acid and cholesterol,so as to provide more theoretical basis for acne vulgaris with Pomegranate Peel Polyphenols ointment,and better applied in clinic.Research methods:The first step:the effective components of pomegranate pomegranate peel polyphenol ointment made of different concentrations,and then select male SPF 40 rats,randomly selected 30,according to Yin Yanru Yin Yanru^[1],for 2 consecutive weeks to 10mg/kg L-arginine by gavage,the rats SD sebaceous gland proliferation,sebum secretion,until after the success of the model is divided into 6 groups:normal control group,model group,ointment group,high,middle and low pomegranate peel polyphenol ointment 5 rats in each group.In the low,middle and high dose group,0.9%,2.4%and 10%pomegranate skin polyphenols ointment were used to coat the shaving area of the rats' back,and the matrix group was coated with ointment matrix.The normal group and the model group were given the same volume distilled water two times a day.After 3 weeks of administration,the weight ratio of sebum was measured by organic solvent extraction and direct weighing method.The second step was to establish an appropriate high-performance liquid chromatography?HPLC?,and calculate the standard curve,the recovery rate and the precision experiment of standard method.The content of linoleic acid?LA?and cholesterol?CHO?in sebum were separated and measured by this high performance liquid chromatography?HPLC?.Results:?1?each animal daily observation,drug coated period,no rats died,the activities of rats in each group is more flexible and more moist hair,stool generally formed elliptical granular applicator parts without desquamation,hyperkeratosis,swelling,skin cracks and other phenomena,the weight changes within three weeks had no significant difference.?2?effects of different concentrations of pomegranate peel polyphenol ointment on sebum sebum secretion rate is expressed as follows:each group:normal control group:?0.3426+0.1417?g/cm2.min;model group:?0.7886+0.1756?g/cm2.min;matrix group:?0.7808+0.1229?g/cm2.min;low dose group:?0.8148+0.1356?in g/cm2.min;dose group:?0.7098+0.2389?g/cm2.min,high dose group?0.4383+0.1273?g/cm2.min,compared with that of model group,the difference was statistically significant?P<0.05?,low,medium and medium group compared with the model group,the difference was not statistically significant?P>0.05?;model group and blank group were statistically significant the difference?P<0.05?.?3?high performance liquid chromatography was established in this study?HPLC?:the column was Supersil ODS column?4.6mm*250mm,5 m?;when the mobile phase of acetonitrile-0.1%phosphoric acid water?A??C??gradient elution,initiation,A:C?95:5??,the separation of linoleic acid flow rate gradient elution with cholesterol,1.0ml/min,wavelength is 210 nm,the column temperature is 30 degrees centigrade.?4?effects of different concentrations of pomegranate peel polyphenol ointment on the content of linoleic acid:normal control group:?0.75+0.078?%;model group:?0.47+0.049?%;matrix group:?0.48+0.110?%;low dose group:?0.44+0.121?%;the middle dose group:?0.43+0.121?%,the high dose group?0.68+0.168?%,compared with that of model group,the difference was statistically significant?P<0.05?,matrix group,low dose group,middle dose group and model group had no statistical significance?P>0.05?.?5?effects of different concentrations of pomegranate peel polyphenol ointment on cholesterol levels:normal control group:?3.39+0.443?%;model group:?3.69+0.790?%;matrix group:?3.73+0.32?%;low dose group:?3.47+0.334?%;the middle dose group:?3.84.0.520?%,high dose group?3.39+0.441?%,the difference was not statistically significant?P>0.05?.Conclusion:1.pomegranate pomegranate polyphenol ointment was used for 3weeks without obvious irritation.2.in this paper,the establishment of HLPC can separate linoleic acid and cholesterol,with high sensitivity and simple operation,which provides more basis for the separation of other components in sebum.3.,high concentration of pomegranate peel polyphenol ointment has the effect of reducing sebum and raising linoleic acid.But it can't be thought that pomegranate polyphenol ointment has influence on cholesterol in sebum.It provides more theoretical basis for acne treatment with external application of pomegranate peel polyphenol ointment.