Construction And Functional Verification Of Abortive Plant Expression Vector

Abortion is one of the hotspots in botanical research.It has been widely used in conventional Crop Breeding.The genetic transformation of abortive plant expression vectors is a simple strategy to induce plant sterility.In this paper,the promoter of Arabidopsis floral meristem and floral organ specific gene API and Coding Region of RNaseH Gene in Escherichia coli were cloned.The expression vector pHZM 13-AP1-RNaseH of abortive plants was constructed.It was introduced into Agrobacterium tumefaciens EHA105 by electroporation.35 transformed strains were obtained from tobacco C151 by Agrobacterium-mediated genetic transerfermation.Pollen tube germination test showed that there was no difference in pollen germination vigor between transerplants and control.The average weight of 100 seeds was 6.6mg for the transformed plants and 9.1 mg for the control plants.It shows that there is a significant difference between themselves.Further scanning of the seed surface by electron microscopy showed that the wrinkle-like ornamentation on the seed coat of the transformed plants decrease.The cause is unknown.Seed germination test showed that the seeds of transformed plants was very low and showed delayed germination.Our work shows that the abortive plant expression vector greatly weakens the fertility of the host.However,suicide genes did not affect floral organogenesis.This may be related to the alteration of the activity of the promoter under the background of heterologous expression.