Aphid Resistance Gene PPA In Arabidopsis Transformation And Validation

In China, agricultural production, prevention and the control of crop aphids mainly use the chemical pesticides, but this approach is too costly and inefficient. Besides, it brings the increasingly human health and environmental pollution problems. Therefore, more and more researchers now are concerned about the applications of insect-resistant crop breeding.Transgenic breeding have the advantages that conventional breeding with general do not have:â‘ broaden the genetic resources that can be applied;â‘¡cultivate high-quality, high yield, high resistance and some other varieties;â‘¢objective traits of plants targeted mutation may occur, or directional selection;â‘£This greatly improves the efficiency of selection and speed up the breeding process. Lectin Pinella (PPA) is separated from Pinella, with clotting activity of the Pinella protein,also has the properties of can specificity mannose-binding and its polymers, to coagulation, anti-tumor, pesticides and other important physiological functions, in recent years has become a hot topic.The topic is main study of that from the Araceae Pinellia genus Pinella cloned a new type of plant lectins (PPA) gene, by approach of Agrobacterium-mediated transformation to import it into this model plant Arabidopsis thaliana, Screened homozygous through several generations, Then put worms by testing, to verify the function of insect-resistant gene.Firstly, use of the genetic engineering technology to clone PPA, experiment through the transformation of plasmid pCAMBIA1380,Makes the PPA in expression vector and have a stronger expression of the 35s promoter activity in dicots and the recent discovery of the fluorescent protein GFP combined, get the following results:â‘ successfully constructed the eukaryotic expression vector driven by 35s promoter gene plant expression vector pCAMBIA1380-35s-PPA-GFP;â‘¡successfully transformed by agrobacterium transfer it to the Arabidopsis thaliana;â‘¢get homozygotes for the follow-up experiments provide an experimental platform for insect.