Expression Patterns And Functional Analysis Of 14-3-3 Genes Related To Stress Resistance Of Haloxylon Ammodendron

Haloxylon ammodendron is an important tree species in desertification control in China.However,desertification and drought stress in desert area are the main ecological limiting factors leading to low survival rate of Haloxylon ammodendron.The optimum Haloxylon ammodendron cultivars were one of the most cost-effective ways to improve the survival rate of transplanting seedlings.The study of the resistance mechanism of Haloxylon ammodendron could alleviate or solve the worsening ecology to a certain extent Environment,China's desertification control and other ecological environment construction is of great significance.In this study,nine 14-3-3 protein genes were cloned from Haloxylon ammodendron,and the promoter of HaFT-7 gene was cloned by Tail-PCR.HaFT-1-HaFT-9 was studied by qRT-PCR The HaFT-3 and HaFT-7 genes were transformed into Arabidopsis thaliana plants by inflorescence infection,and the resistance to Arabidopsis thaliana transgenic HaFT-3 and HaFT-7 genes was studied analysis.The main findings are as follows:1.The promoter sequence of Haloxylon ammodendron HaFT-7 gene was obtained by Tail-PCR with a size of 1559 bp,and the sequence was predicted by online software.It was found that the HaFT-7 promoter was involved in the photoresponsive elements such as Box 4,CATT-motif,Sp1 and so on.Elements such as MBS,MYCATERD1 and so on were also predicted for the MYB binding site,such as LTR,ARE Etc;also includes ABRE,SARE and other hormonal components,indicating that the promoter,including response to light,plant hormones,drought and other adversity of the cis-acting elements,and plant growth and development related to the regulatory elements.2.The expression of 9 14-3-3 protein gene was detected by fluorescence quantification at 20% PEG6000 simulated drought,high temperature and salt stress,and IAA and ABA treatment.The results showed that the nine genes were able to cope with stress,but they were different in drought,high salt,high surface temperature,ABA and IAA treatment.3.The pCAMBIA1304-HaFT-3 and pCAMBIA1304-HaFT-7 plant expression vectors were successfully constructed.The target gene was transferred into Arabidopsis thaliana by inflorescence infiltration,identified by multi-generation screening and selected by semi-quantitative analysis.The transgenic Arabidopsis thaliana plants with high gene expression were analyzed for stress resistance.The transgenic Arabidopsis thaliana and wild type Arabidopsis thaliana plants were selected for drought and high temperature,and the resistance of transgenic Arabidopsis thaliana was analyzed from phenotypic and physiological indexes.The results showed that the genotypes of two transgenic Arabidopsis were superior to non-transgenic plants under drought and high temperature stress,and the survival rate and biomass of Arabidopsis thaliana under high temperature stress were better than that of HaFT-7.The HaFT-3 was more resistant to high temperature,and the accumulation of proline was higher than that of wild type Arabidopsis thaliana after stress.Under salt stress,the content of malondialdehyde in transgenic plants was slower than that of wild type.Comprehensive analysis,transgenic Arabidopsis thaliana for drought,high temperature and salt stress has a stronger resistance.