Research Of Glucose Sensor Based On Printing Technology

In this thesis,glucose sensor is fabricated by printing method.The glucose sensor without restriction of enzyme activity,good integration,wide detection range,good stability and low production cost was produced.The composite materials with catalytic activity to glucose were prepared by reduction of nickel and copper oxide grown on the surface of carbon nanotubes by in situ reduction method.The electrode of the sensor is printed by screen printing,and the working electrode,the contrast electrode,the reference electrode are integrated into the same test strip.The composite catalytic material was modified to the reaction area of the working electrode and connected to the electrochemical workstation to produce a printed electrode glucose sensor.At the same time,the catalytic composite materials and surface morphology were characterized,then the conductivity of the printed electrodes were tested,then the electrochemical behavior of printing electrode sensor and the detection performance of glucose test,finally,and comparative analysis for sensor glassy carbon electrode.The contents are as follows:1.Nickel and carbon nanotube composites,copper oxide and carbon nanotube composites were prepared by in situ reduction method.By Fourier transform infrared spectroscopy between carbon nanotubes and acidified carbon nanotubes treated,the carbon nanotube surface oxygen functional groups increased after acidification;through the XRD to detect composite catalytic material,nickel oxide and copper successfully grown on the surface of carbon nanotubes;through the SEM inspection of composite materials,and nickel particles uniformly grown on the surface of carbon nanotubes,and copper oxide is grown on the surface of carbon nanotubes in a needle like shape.2.Useing screen printing technology,the working electrode(conductive carbon paste),the contrast electrode(conductive carbon paste)and reference electrode(Ag/AgCl mixed ink),printing guide(conductive silver paste),an insulating layer(insulating ink printing)printing on PET substrate to fabrication of the sensor electrode.The conductivity of printed electrode was tested by four point resistance tester.The working electrode and the electrode compared the average resistivity is 2.02 ?·cm standard deviation was 0.0853;the average printing guide resistivity is 6.1 m?·cm standard deviation is 0.6603.The printed electrode has good conductivity,which can meet the requirements of sensor electrode.3.The catalytic materials were modified to the reaction region of the printed electrode and the glassy carbon electrode by chemical modification,and then connected to the electrochemical workstation to produce the printed electrode sensor and the glassy carbon electrode sensor.The electrochemical behavior and glucose detection performance of printed electrode sensors and glassy carbon electrode sensors were tested by cyclic voltammetry and chronoamperometry.The nickel composite material of catalytic activity and conductivity than copper oxide composite;printing electrode sensor and glass carbon electrode sensor has good detection performance for glucose,in addition to other sensors outside the printing electrode modified with copper oxide composites have very good stability,and the detection range of glucose was greater than 10mM,and the response time was 10?15s,the detection range of glucose covering the body the blood glucose concentration range,and can be used in the actual measurement of blood glucose.