Study On Brain Image Segmentation And Three-dimensional Reconstruction For The Construction Of The Carp Brain Atlas

The animal robot has become the frontier interdisciplinary field and has attracted much attention.It takes animal as the carrier,using brain control technology which implants electrode in animal brain motor functional areas replacing people in harsh or dangerous environments to complete works concerned in accordance with human wishes or instructions.The practice shows that the brain atlas is an important tool to provide information of the position and function of the brain motor nucleus.It plays an important role in the control of animal robot.The carp was used as the studying object.According to the characteristics of the carp skull structure,the middle point of the first scale rear edge of the junction between the head and the trunk is determined as the coordinates origin to establish the threedimensional coordinate system of the brain,and the spatial position of the brain tissue of the carp in the cranial cavity can be certain with the stereotaxic apparatus.Through the perfusion technique of the carp,the brain specimens were pre-fixed and the fixed brain of which were intact and well-structured.Paraffin-embedded brain tissue of carp was prepared and stained with HE staining.Images were collected using stereo microscope and PC to obtain the original color image of carp brain slices.In terms of image segmentation,the mapping relationship between the brain tissue morphology and the stained brain slice color information was established in this paper.Division of neuron areas with neurons and nerve fibers was completed according to the difference of brightness.Separation of dense nerve fiber regions,mixed regions of neurons and nerve fibers was finished using color difference obtain the clear segmentation result.In terms of image registration,this paper studied in depth in different brain region registration algorithms combined with the brain tissue slice images of carp.Using a registration algorithm based on internal feature points to register the images of cerebellar coronal slices.Using the edge feature point-based registration algorithm to register the images of coronal,midbrain and cerebellum petal coronal slices.Using a registration algorithm based on edge feature points to register the images of axial,midbrain andcerebellar axial slices of carp.Registration results can meet the registration requirements.The GUI was designed to combine the image processing programs involved with the segmentation and registration algorithms,making the image processing operations more intuitive,integrated and systematic.In terms of three-dimensional reconstruction,this paper chose the Visualization Tookit,combining the Marching Cubes and ray casting algorithms to perform surface reconstruction and volume rendering of the main nucleus and nucleus cluster area of the carp brain.Furthermore,the 3D-DOCTOR was used to reconstruct the internal nerve nuclei of diencephalon,midbrain and cerebellum valves,which lays solid foundation for the construction of carp brain atlas.