Preparation And Application Of A Conjugated Polymer Fluorescence Sensor For Continuous Recognition Of Copper And Pyrophosphate

Recently,due to the pervasive application of transition metal ions and anions in industry,biochemistry,medicine,environment and other fields,more and more attention has been paid to their detection.Among them,copper,as the third most abundant transitional metal element in human body,is involved in many biological activities,such as regulating metabolism,building calcium in bone,important component of enzymes and proteins and so on.However,the disorder of copper content could lead to many diseases.Therefore,it is necessary and important to monitor the content of Cu²⁺in the human body.Among many anions,pyrophosphate ion?PPi?,as an important biological anion in the human body,is often released in the hydrolysis of ATP and chain reaction of DNA polymerase,and is involved in many life activities,such as RNA and DNA replication,metabolism,inhibition of calcification and so on.The content of PPi in the body fluid is very plentiful,such as synovial fluid,blood serum and urine.But the imbalance of PPi content in the body could also lead to many diseases.At present,the detection of PPi has become a real-time DNA sequencing method and an important topic of cancer research.Besides,it has been reported that the detection of PPi in synovial fluid has become an important indicator for the diagnosis of arthritis,chondrocalcinosis,calcium pyrophosphate deposition and other diseases.Therefore,the detection of PPi in the body fluid is also very important.At present,there are many methods for the detection of Cu²⁺and PPi,such as atomic absorption spectrometry,atomic emission spectrometry,high performance liquid chromatography,electrochemical analysis,UV colorimetric analysis,fluorescence spectroscopy and so on.Among these,due to low cost,simple operation,fast analysis,wide linear range,low detection limit,high sensitivity,fluorescence spectroscopy stood out.In recent years,conjugated polymer fluorescence sensor has become a hot topic,because of the significant amplification of fluorescence signal by the unique?-?*conjugated molecular wire structure of conjugated polymer.At present,the fluorescence sensors for separate identification of Cu²⁺and PPi have been widely reported;but for continuous identification of Cu²⁺and PPi in the same system were still rarely developed;in which conjugated polymer fluorescence sensors were almost no more.Therefore,a conjugated polymer fluorescence sensor PPE-DPA was designed and synthesized in this thesis,and the continuous detection of Cu²⁺and PPi in the same system was successfully realized.In the first chapter,it was summarized that these important roles and detection methods of Cu²⁺and PPi,and the research progress of fluorescent chemical sensors which detect Cu²⁺and PPi continuously.In the second chapter,the design idea and synthetic steps of PPE-DPA were introduced,and the products of each step were characterized.In the third chapter,it was studied that the specific identification and detection of Cu²⁺by PPE-DPA,and the conditions of the test system were optimized.Under the optimal conditions,the spectral titration of Cu²⁺to PPE-DPA was carried out.The linear detection concentration range was 0.05-5.0?mol/L,and the detection limit was low to 24 nmol/L.In the fourth chapter,the selectivity and anti-interference ability of PPE-DPA-Cu²⁺system to PPi was discussed,which realized the continuous detection of Cu²⁺and PPi successfully.And in the titration experiment of PPi,it was concluded that linear detection concentration range is 0.5-12.0?mol/L,the detection limit as low as 230 nmol/L.Finally,the PPE-DPA-Cu²⁺system was applied to the quantitative detection of PPi in synovial fluid and blood serum.The results were consistent with the literatures and the recoveries were good,which proved the practical application ability of PPE-DPA.