| Acute high-intensity exercise induces oxidative stress in the body,which can cause great damage to the body.Astaxanthin is a very pow erful antioxidant.In order to explore the effects and pathways of astax anthin on oxidative stress induced by acute high-intensity exercise,this study investigated whether astaxanthin supplementation through Nrf2 wa s based on a model of skeletal muscle injury induced by oxidative stre ss induced by acute acute high-intensity exercise.Enhance the body's a ntioxidant capacity while offsetting part of the oxidative stress injury c aused by acute high-intensity exercise.The subjects of this experiment were 7-week-old male SD rats,we ighing 180-200 grams.The experimental animals were raised,fed and exercised in the animal room of Shanxi University.Rats were divided i nto 4 groups after 5 days of adaptive breeding.They were the control group(group C);the simple administration group(group M);the exerci se alone group(group E);the exercise plus administration group(EM group).In the following 4 weeks,the astaxanthin was administered to t he M and EM groups at a dose of 25 mg/kg per day,and the C an d E groups were administered to the soybean oil.On the first day afte r the rats were grouped,each group of rats was subjected to adaptive exercise training,and on the 20th day after the group,the incremental load exercise test was performed again.The average exhaustion speed of the rats was 21 m/min(75%V02max).On the 29th day,the E and EM groups performed the final one-time high-intensity exercise.Th e rats in group E and EM were on a running platform with a gradient of-10°,and the speed was 24 m/min.Each rat exercised 8 groups,the exercise time was 5 minutes,and each group was intermittent for 1 minute.Rats were sacrificed immediately after exercise.Blood and g astrocnemius were taken from the rats.Plasma blood lactic acid,SOD,MDA,and GSH levels in each group were measured immediately after exercise.The relative protein expression of p-Akt,Nrf2,and HO-1 in gastrocnemius(/β-actin)and mRNA expression levels of Akt,Nrf2 an d HO-1 in rat gastrocnemius muscle.The results showed that:in the quiet state,the levels of blood lac tic acid and plasma MDA in group M were lower than those in group C,and the plasma SOD activity was higher than that in group C,all r eaching extremely significant levels(P<0.01);at the molecular level,p-Akt and HO in group M The relative expression of-1 was significantl y higher than that of group C(P<0.01),and the mRNA expression of HO-1 was also higher than that of group C,reaching a significant lev el(P<0.05).Immediately after the acute high-intensity exercise,the le vels of blood lactic acid,SOD,and MDA in group E were significantl y higher than those in group C(P<0.01).The GSH level in group E was extremely significantly lower than that in group C(P<0.01).At t he molecular level,the relative expression of Nrf2 protein in group E was significantly lower than that in group C(P<0.01).The levels of plasma lactic acid and MDA in the EM group were significantly lower than those in the E group(P<0.01),and the relative protein expressi on levels of p-Akt,Nrf2,and HO-1 in the EM group were significantl y higher than those in the E group(P<0.05).The expression of HO-1 mRNA in the group was significantly higher than that in the E group(P<0.05).Conclusions:1.Acute high-intensity exercise can cause oxidative s tress in the body,and also has a certain stimulating effect on anti-oxid ase.2.Astaxanthin supplementation can significantly improve the level of bone lipid peroxidation in rats and inhibit oxidative stress.3.Astaxa nthin activates Akt and further activates Nrf2,increasing the expression of downstream HO-1,thereby effectively inhibiting oxidative stress ca used by exercise. |
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