The Expression Levels And The Significance Of DLL1 And Its Receptor Notch3 In Oral Lichen Planus Lesions

Objective:To explore the expression of Delta-like-1（DLL1）and its receptor Notch3 in oral lichen planus（OLP）lesions and their relationship with interferon-gamma（IFN-γ）,to explore the role and significance of DLL1 and its receptor Notch3 in the formation of local lesions of OLP.Methods:40 samples of OLP lesions（20 samples with erosive type and 20 samples with non-erosive type）and16 samples of healthy oral mucosa were used as controls.Real-time fluorescent quantitative PCR（q PCR）was used to detect the m RNA expression levels of DLL1,Notch3and IFN-γin lesions.The distribution and expression of DLL1,Notch3 and IFN-γin tissues were detected by immunohistochemistry,and the relationship between the exprssion of DLL1,Notch3 and IFN-γin OLP lesions and their relationship with the clinicopathological characteristics of OLP were analyzed.Results:（1）The expression levels of DLL1,Notch3 and IFN-γm RNA in OLP lesions were higher than those in the normal control group（P<0.05）,but there was no significant difference between the two OLP types（P>0.05）;（2）The expression levels of DLL1,Notch3 and IFN-γprotein in OLP lesions were higher than those in the normal control group（P<0.05）,and the expression of DLL1,Notch3 and IFN-γwere positively correlated(r_s1=0.390,P₁<0.05;r_s2=0.320,P₂<0.05),the expression of DLL1 was positively correlated with the expression of Notch3（r_s=0.395,P<0.05）;Further analysis of the relationship between DLL1,Notch3,IFN-γand the clinicopathological characteristics of OLP,the results showed that the positive expression rate of DLL1,IFN-γin OLP was positively correlated with the degree of basal cell liquefaction(r_s1=0.387,P₁<0.05;r_s2=0.383,P₂<0.05),there was no significant difference in the expression of DLL1,Notch3 and IFN-γbetween the two OLP types（P>0.05）.Conclusion:The abnormal expression of DLL1,Notch3 and IFN-γin local lesions of OLP suggests that DLL1,Notch3 and IFN-γmay jointly participate in the pathological damage process of OLP.