Effects Of Allicin Combined With Paclitaxel On Expression Of CD44, CXCL12 And NCAM In ACC-M Cells Of Salivary Adenoid Cystic Carcinoma

Objective:To study the effects of allcin combined with paclitaxel on the proliferation inhibition and apoptosis induction of ACC-M cells in salivary adenoid cystic carcinoma,and exploring the effects of allicin combined with paclitaxel on the expression of CD44,CXCL12and NCAM proteins in ACC-M cells,so as to provide theoretical and experimental basis for experiments and clinical applications of anti-tumor drugs in the future.Methods:There were four groups,allicin group?allicin?,paclitaxel group?PTX?,allicin combined with paclitaxel group?allicin+PTX?and control group respectively.The control group was treated with 0.1%DMSO in PBS.?1?Cck-8 experimental design:the concentration gradient of garlicin was set as?20?g/ml,40?g/ml,60?g/ml,80?g/ml,100?g/ml?,and the concentration gradient of paclitaxel was set as?5?g/ml,10?g/ml,20?g/ml,40?g/ml,60?g/ml?.The proliferation inhibition rate of ACC-M cells?24h,48h,72h?with above different gradients was detected.After the experimental data were collected,the half maximal inhibitory concentrations of allicin and paclitaxel for 48 hours were calculated respectively by SPSS 20.0.The proliferation inhibition rates of allicin semi-inhibitory concentration group,paclitaxel semi-inhibitory concentration group,allicin semi-inhibitory concentration combined with paclitaxel semi-inhibitory concentration group on ACC-M cells for 48 hours were detected.?2?The apoptosis rate of ACC-M cells treated with different concentrations of drugs after 24 and 48 hours was detected with flow cytometry.?3?Western blot was used to respectively detect the expression levels of CD44,CXCL12 and NCAM protein in tumor cells after the treatment with IC₅₀0 paclitaxel and IC50 paclitaxel for 48 hours.?4?RT-PCR was used to detect the expression level of CD44,CXCL12,and NCAM mRNA in ACC-M cells treated with different drug groups for 48 hours.Results:?1?Wthin the same period of time,with the concentration of allicin and paclitaxel increased,the proliferation inhibition rate of ACC-M cells increased.And the cell inhinition rate has positive correlation with the drug concentratio?P<0.05?.?2?After the treatment of the same concentration of allicin and paclitaxel,the inhibition of cell proliferation was enhanced by extending hours.?3?Compared with the allicin IC50 and paclitaxel IC50 group,the inhibition rate of cell proliferation in allicin combined with paclitaxel group was significantly higher.Allicin and paclitaxel have synergistic effect?F=779.469,P<0.05?.?4?Flow cytometry showed that allicin and paclitaxel could induce apoptosis of ACC-M cells,allicin combined with paclitaxel group had a stronger effect on the apoptosis of ACC-M cells than the drug alone,and the difference was statistically significant?P<0.05?.?5?Both allicin and paclitaxel could down-regulate the expression of CD44,CXCL12 and NCAM protein in ACC-M cells,moreover the regulation of target protein was the most significant when allicin was combined with paclitaxel?P<0.05?.?6?The relative expression of CD44,CXCL12 and NCAM mRNA in ACC-M cells was downregulated by allicin combined with paclitaxel alone and in combination.?P<0.05?Conclusion:?1?The inhibition rate of ACC-M cell proliferation by allicin and paclitaxel increased with the increase of concentration.allicin combined with paclitaxel can enhance the apoptotic efficiency of ACC-M cells and reduce the dosage of paclitaxel.?2?Allicin,paclitaxel and both of them could downregulate the expression of CD44,CXCL12 and NCAM protein in ACC-M cells.