Study On Plasma Long-noncoding RNA-MIAT And MicroRNA-181b In Patients With Acute Coronary Syndrome

Background:Acute coronary syndrome(ACS)is one of the most severe disease in clinic.Epidemiology shows that ACS is characterized by high morbidity and mortality worldwide.Early identification of ACS high-risk groups,early diagnosis and treatment can significantly improve the prognosis of myocardial infarction and reduce the risk of death.nc RNAs were considered as “evolutionary junk”,but increasing evidence suggests a huge impact on several molecular mechanisms.Micro RNAs(mi RNAs/mi R)with a size of 19-25 nucleotides are the most known group of nc RNAs.Micro RNAs are reported to play a key role in the development of cardiac disease.Studies have shown that mi R-181 b can relieve inflammatory responses and reduce atherosclerotic lesion formation.Lnc RNAs,a group of transcribed RNA molecules with no or limited of protein-coding potential,are mainly generated by RNA polymerase(Pol)II/Pol I.Recent studies have shown that Lnc RNAs plays an important role in cardiac development and cardiovascular disease.Myocardial infarction-associated transcript(MIAT),a conserved mammalian lnc RNA,was first identified in 2006.Studies have shown that serum MIAT levels in patients with coronary heart disease remained at a relatively high level and the serum MIAT concentration was positively correlated with the concentrations of IL-6 and TNF-?.Study shows that mi R-181 b was a potential target of MIAT.Both mi R-181 b and MIAT can regulate the inflammatory process by regulating inflammatory factors.However,the role of MIAT and mi R-181 b in the pathogenesis of ACS are still unclear.Objective:In this study,the expression levels of MIAT and mi R-181 b in peripheral blood of ACS group and control group were detected and evaluated the correlation of MIAT and mi R-181 b in peripheral blood of ACS group,then we anlysized the potential role of MIAT and mi R-181 b in the diagnosis and treatment of patients with ACS and explore their possible mechanism in the pathogenesis of ACS.Methods:116 patients from Department of Cardiology,the Second Hospital of Ji Lin University from December 2018 to November 2019 were enrolled in this study.Among them 72 patients were in ACS group.After the peripheral venous blood was collected,we measured levels of MIAT and mi R-181 b in plasma by real-time polymerase chain reaction.The statistical analyses were performed using SPSS25.0.The receiver operating characteristic curve(ROC)was used to evaluate the predictive power,and the area under the ROC curve(AUC)was used to estimate the sensitivity and specificity as well as the best cut-off value for the selected variable.The correlation analysis was undertaken with the linear correlation analysis.Results:1.The expression levels of plasma MIAT and mi R-181 b between ACS and the control group was analyzed.The results showed that,compared with the control group,plasma MIAT expression levels in the ACS group were significantly up-regulated and mi R-181 b expression levels were significantly down-regulated(P<0.05).2.In subgroup of the ACS group,the expression levels of MIAT and mi R-181 b in the STEMI groups and NSTEMI groups were higher than those in the UA group(P <0.05).There was no significant difference in the expressions levels of mi R-181 b and MIAT among the STEMI gruop and NSTEMI group(P>0.05).3.There was no significant difference in the expressions levels of MIAT and mi R-181 b among the single ACS group,ACS with hypertension and diabetes group,ACS with hypertension group and ACS with diabetes group(P>0.05).4.There was a negative correlation between MIAT and mi R-181 b in plasma of patients with ACS.And there was a negative correlation between MIAT and mi R-181 b in plasma of patients with MI and UA.5.The ROC analysis showed that the AUC of MIAT was 0.946(95%CI :0.909-0.983;P<0.001),the optimal cut-off value was 3.06(sensitivity: 79.2%;specificity: 95.5%).The AUC of mi R-181 b was 0.884(95%CI: 0.822-0.945;P<0.001),the optimal cut-off value was 0.46(sensitivity: 84.7%;specificity: 84.1%).Conclusion:The expression levels of plasma MIAT in the ACS group were significantly up-regulated and mi R-181 b expression levels were significantly down-regulated.There was a negative correlation between MIAT and mi R-181 b.The expression level of MIAT in plasma predictive potency for ACS,might be as additional biomarker for ACS.mi R-181 b has a certain predictive power on the diagnosis of ACS and the severity of the disease.