| Research background and purpose:Psoriasis is a chronic inflammatory disease mainly damaging external skin,the etiology is still unclear,it is generally believed that many factors,including genetics,environment and immunity,interact with each other,leading to the occurrence and exacerbation of psoriasis.At present,psoriasis is easy to repeat and cannot be cured.Therefore,the pathogenesis of psoriasis has become an urgent problem.T cells palys a prominent role in the development of psoriasis,especially helper T cells and cytokines related.IL-23/IL-17 axis and TNF-α/IFN-r axis have been proved to be important pathway in the disease process,of them,IL-17 and TNF-α are important proinflammatory cytokines in psoriasis,as well as the newly-found IL-9.PU.1 and IRF4 are important transcription factors of immune cells,they all contribute to the differentiation,development and proliferation of myeloid and lymphoid lineages,and may play critical roles in immune dysfunction and inflammation.To date,they were mainly found in autoimmune diseases and tumors,while few is found in psoriasis.Clarify the differential expression of PU.1,IRF4 and pro-inflammatory cytokines like IL-9,IL-23,IL-17,IFN-r,TNF-α in psoriasis vulgaris,define the correlation with psoriasis area and severity index(PASI score),and explore the possible correlation with the pathogenesis of psoriasis to provide reference for the following study.Materials and Methods:1.51 patients with psoriasis vulgaris and 25 healthy subjects were involved according to the entry criteria;2.10 ml of peripheral blood were withdrawn from patients and controls included,the plasma and blood cells were separated,the cytokines IL-9,IL-17,IL-23,IFN-r,TNF-α expression in plasma were detected by enzyme-linked immunosorbent assay(ELISA);3.CD4+ T cells were separated,and determine the gene expression with Real Time Quantitative Polymerase Chain Reaction(qRT-PCR);4.Compare the differential expression of PU.1,IRF4 and cytokines of two groups,determine the correlation of PU.1,IRF4 with cytokines related and PASI score.Result:1.The expression of PU.1,IL-9,IL-17,IL-23,IFN-r and TNF-αmRNA in psoriatic patients were all higher than those in controls,and PU.1,IL-17,IFN-r and TNF-α were statistically significant(P<0.05);while IRF4 was significantly lower(P<0.05).2.The IFN-rmRNA in case group was positively correlated with PASI score(P<0.05).3.The PU.1mRNA vulgaris was positively correlated with gene expression of IL-17,IFN-r and TNF-α in group with psoriasis(P<0.05),IRF4 mRNA was negatively correlated with IL-17 mRNA,but positively correlated with IL-23 gene expression.4.Cytokines IL-9,IL-17,IFN-r and TNF-α in plasma of case group is significantly higher than those in control group(P<0.05).5.There was no correlation of cytokines IL-9,IL-17,IL-23,IFN-r and TNF-α in plasma with PASI score(P>0.05).6.IRF4 mRNA was positively correlated with IL-17 in plasma(P<0.05);while PU.1mRNA was not correlated with the cytokines(P>0.05).Conclusion :1.The expression of PU.1 mRNA was increased in the psoriasis vulgaris group,while the expression of IRF4 mRNA was down-regulated,and the significant differential expression suggested that they may be involved in the inflammatory response or immune disorder process of psoriasis.2.The cytokine expression trends in the IL-23 / IL-17 pathway and TNF-α / IFN-r pathway are consistent with previous literature,confirming their important role in the pro-inflammatory mechanism in psoriasis once again.3.PU.1 has a positive correlation with the pro-inflammatory genes IL-17,IFN-r and TNF-α,suggesting that it may participate in psoriasis through acting on the IL-23 /IL-17 pathway,TNF-α / IFN-r pathway,etc.4.IRF4 is positively correlated with the cytokine IL-17 concentration in plasma,positively correlated with IL-23 mRNA,and negatively correlated with IL-17 mRNA,indicating that IRF4 may directly affect Th17 cells or indirectly promote IL-17 secretion through IL-23. |
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