The Expression And Significance Of RANKL?p-ERK1/2 And P-mTOR In Extramammary Paget's Disease

Objective:To detect the expression level of RANKL,p-ERK1/2 and p-mTOR in extramammary Paget's disease and to observe and evaluate the role of cellular signaling pathway in the pathogenesis of EMPD and its biological significance.Methods:From 2008 to 2017,80 patients with EMPD were selected from Dermatology Department of the First Affiliated Hospital of Fujian Medical University and Fujian Dermatology Hospital.All of them were diagnosed by clinical and pathological methods.The expression of RANKL,p-ERK1/2 and p-mTOR in EMPD and its expression pattern were observed by immunohistochemical method.The dyeing rate and intensity were calculated,the data were processed by statistical software,and the correlation and significance of each index were analyzed.Result:1.RANKL was localized in Paget cytoplasm.66 cases were positive and 14 cases were negative.The positive rate was 82.5%.The p-ERK1/2 was localized in Paget cytoplasm.69 cases were positive and 11 cases were negative.The positive rate was 86.25%.The p-mTOR was also localized in cytoplasm,71 cases were positive,9 cases were negative,the positive rate was 88.75%.2.In EMPD,in the expression correlation coefficients of RANKL,p-ERK1/2,p-mTOR were r = 0.907,p < 0.05,r = 0.931,p < 0.05,r = 0.98,p < 0.05,They are positively correlated.Conclusion:1.The high expression of RANKL.p-ERK1/2.p-mTOR suggest that RANK-RANKL signal pathway,RAS-RAF-MEK-ERK signal pathway and PI3K-AKT-mTOR signal pathway are activated in EMPD,and play an important role in the occurrence and development of this disease.2.There is a positive correlation among the expression of RANKL?p-ERK1 / 2?p-mTOR,which suggests that the signaling pathway is related to each other and promotes the growth,proliferation and metastasis of tumor cells,and increases the invasiveness of the disease.3.The further study of the three signaling pathways will be helpful to explain the pathogenesis of EMPD and provide a new theoretical basis for the development of tumor targeting drugs.