Prrx1 Regulates EMT Process In Gastric Cancer Through The P53 Pathway

Objective To investigate the relationship between the expression of Prrx1 and the clinicopathological characteristics,epithelial-mesenchymal transition markers and p53 proteins in gastric cancer and adjacent tissues.And the effect of Prrx1 on the factors related to EMT and the p53 pathway in gastric cancer cells.To explore whether Prrx1 regulates EMT process in gastric cancer through the p53 pathway.Methods 1.Collected 89 cases of clinical gastric cancer and adjacent tissue samples.Immunohistochemistry staining was used detect the expression of Prrx1,EMT markers,p53 in gastric cancer and adjacent tissues,and analyzed the relationship between Prrx1 and the clinicopathological characteristics,EMT markers and p53.2.RT-qPCR and Western blot were used to detect the expression of Prrx1 in gastric cancer MNK45,SGC7901 cells and normal gastric mucosa GES-1 cells.3.After overexpression and interference of Prrx1 with lentivirus transfection of gastric cancer MNK45 and SGC7901 cells,RT-qPCR and Western blot methods were used to detect the expression of Prrx1,EMT markers and p53 pathway related factors.After added p53 pathway inhibitor Pifithrin-α to MNK45 Prrx1 overexpressing cells,RT-qPCR and Western blot were used to detect the mRNA and protein expression of p53 pathway related factors,Prrx1 and EMT markers;and scratch test and transwell experiment were used to detect the migration and invasion capacity of gastric cancer cellsResults 1.The positive rate of Prrx1 protein in gastric cancer tissues was significantly higher than that in adjacent tissues,and it was significantly related to the depth of tumor invasion,lymph node and distant metastasis,TNM stage of patients(P<0.05),but not related with age and gender of the patient,tumor size and differentiation(P>0.05).Further analysis revealed that the expression of Prrx1 was negatively correlated with the expression of E-cadherin and p53,while positively correlated with the expression of Vimentin.2.The mRNA and protein expressions of Prrx1 were low expressed in normal gastric mucosal GES-1 cells,but high expressed in gastric cancer MNK45 and SGC7901 cells.3.After Prrx1 overexpression in MNK45 cells,the mRNA and protein expression of Vimentin and bcl-2 were significantly higher than those in the control group,while E-cadherin,p53 and Bax were significantly reduced(P<0.05).After Prrx1 interference in SGC7901 cells,the mRNA and protein expressions of Vimentin and bcl-2 were significantly lower than those in the control group(P<0.05),while E-cadherin,p53 and Bax were significantly increased(P<0.05).The mRNA and protein expressions of p53,Bax and e-cadherin were significantly decreased after Pifithrin-α of p53 signaling pathway inhibitor was added to MNK45 Prrx1 overexpressed cells(P<0.05),while bcl-2 and Vimentin were significantly increased(P<0.05),but Prrx1 showed no significant change(P<0.05),and the migration and invasion capacity increased significantly(P<0.05).Conclusion The high expression of Prrx1 in gastric cancer tissues is correlated with the invasion and metastasis of gastric cancer,and is negatively correlated with E-cadherin and p53,while positively correlated with Vimentin.Prrx1 can induce EMT in gastric cancer cells and affect the expression of the p53 signaling pathway related factors.Prrx1 regulates the EMT process of gastric cancer cells by inhibiting the p53 pathway.